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Ultrasensitive DNA Detection by Cascade Enzymatic Signal Amplification Based on Afu Flap Endonuclease Coupled with Nicking Endonuclease

2010; Wiley; Volume: 50; Issue: 32 Linguagem: Inglês

10.1002/anie.201001529

1521-3773

Bingjie Zou, Yinjiao Ma, Haiping Wu, Guohua Zhou,

CRISPR and Genetic Engineering

Hail CESA! Cascade enzymatic signal amplification (CESA) for DNA detection is achieved in three steps: invasive signal amplification by Afu endonuclease to generate amplified flaps, flap ligation by T4 ligase to form a nicking site, and nicking reaction by nicking endonuclease to produce amplified signals (see picture for last step). The sensitivity of CESA is as high as 1 fM DNA.