Regulation of nuclear poly(A) addition controls the expression of immunoglobulin M secretory mRNA
2001; Springer Nature; Volume: 20; Issue: 22 Linguagem: Inglês
10.1093/emboj/20.22.6443
ISSN1460-2075
Autores Tópico(s)RNA Research and Splicing
ResumoArticle15 November 2001free access Regulation of nuclear poly(A) addition controls the expression of immunoglobulin M secretory mRNA Catherine Phillips Corresponding Author Catherine Phillips Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Stephen Jung Stephen Jung Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Samuel I. Gunderson Samuel I. Gunderson Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Catherine Phillips Corresponding Author Catherine Phillips Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Stephen Jung Stephen Jung Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Samuel I. Gunderson Samuel I. Gunderson Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA Search for more papers by this author Author Information Catherine Phillips 1, Stephen Jung1 and Samuel I. Gunderson1 1Rutgers University, Nelson Labs Room A322, 604 Allison Road, Piscataway, NJ, 08854 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2001)20:6443-6452https://doi.org/10.1093/emboj/20.22.6443 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info B-cell differentiation is accompanied by a dramatic increase in cytoplasmic accumulation and stability of the IgM heavy chain (μ) secretory mRNA. Despite considerable effort, the mechanism is unknown. We have identified three short motifs upstream of the secretory poly(A) site, which, when mutated in the μ heavy chain gene, significantly increase the accumulation of the secretory form of poly(A)+ mRNA relative to the membrane form and regulate the expression of the secretory poly(A) site in a developmental manner. We show that these motifs bind U1A and inhibit polyadenylation in vitro and in vivo. Overexpression of U1A in vivo results in the selective inhibition of the secretory form. Thus, this novel mechanism selectively controls post-cleavage expression of the μ secretory mRNA. We present evidence that this mechanism is used to regulate alternative expression of other genes. Introduction The poly(A) tail plays an important role in post-transcriptional control of gene expression in regulating mRNA transport, stability and translation (Ross, 1995; Gallie, 1998; Preiss and Hentze, 1999). Poly(A) tails are formed in the nucleus in conjunction with RNA splicing and 3′ end cleavage and, with the exception of histone mRNA, mRNAs that are not polyadenylated correctly are not transported to the cytoplasm and are degraded rapidly (Bousquet-Antonelli et al., 2000; Le Hir et al., 2000; Shatkin and Manley, 2000; Tollervey and Caceres, 2000; reviewed in Minvielle-Sebastia and Keller, 1999; Wahle and Ruegsegger, 1999; Zhao et al., 1999). The IgM heavy chain (μ) pre-mRNA is processed alternatively to either a membrane or a secretory mRNA by alternative poly(A) site usage, which is regulated during B-cell development (Early et al., 1980; Galli et al., 1988; Perry et al., 1988; Peterson and Perry, 1989) (Figure 1A). This is accompanied by dramatic changes in total μ mRNA accumulation, which is not accounted for by an increase in transcription rate as the heavy chain enhancer is fully active even in pre-B cells (Gerster et al., 1986). These changes can be explained by changes in μ mRNA stability and nuclear to cytoplasmic transport (Darnell, 1982; Berberich and Schimpl, 1990). It has been shown that the half-life of μ mRNA roughly doubles from 3–8 to 14–16 h during differentiation (Mason et al., 1988; Cox and Emtage, 1989; Berberich and Schimpl, 1990; Reed et al., 1994). Perry and Kelley (1979) found a 6-fold change in total μ mRNA nuclear levels compared with a 150-fold change in cytoplasmic levels between 70Z/3 (a pre-B cell line) and MPC11 (a myeloma). Figure 1.Schematic model of the immunoglobulin secretory poly(A) site. (A) The genetic organization of the IgM heavy chain and its alternative processing to a secretory or a membrane form of mRNA. (B) The location of the secretory poly(A) site and relative location of the 5′ splice site, the U1A-binding motifs, the hexanucleotide sequence and the downstream GU-rich regions. Numbers indicate the positions of the ends of the RNA substrates and plasmid inserts referred to in the text. (C) The length and position of the antisense probe used for RNase protection assays and the protected fragments for the μ secretory and μ membrane mRNA, respectively, referred to in Figures 6 and 7. Download figure Download PowerPoint Using lipopolysaccharide-stimulated primary B cells, Berberich and Schimpl (1990) distinguished μ secretory mRNA and μ membrane mRNA and found that the doubling of the half-life was due to a change in secretory μ mRNA stability as the half-life of the membrane form did not change. They found no change in the ratio of secretory to membrane mRNA accumulation in the nucleus but a drastic shift to μ secretory mRNA in the cytoplasm. These results suggest that mechanisms responsible for the increased stability and nuclear to cytoplasmic transport make a greater contribution to the increased expression of the μ secretory mRNA during B-cell differentiation than an up-regulated usage of the μ secretory poly(A) cleavage site. In recent years, much work has focused on the regulation of cleavage efficiency at the secretory poly(A) site (Peterson and Perry, 1989; Peterson, 1992; Edwalds-Gilbert and Milcarek, 1995; Phillips et al., 1996, 1999; Phillips and Virtanen, 1997; Martincic et al., 1998; Takagaki and Manley, 1998; Veraldi et al., 2001). However, the molecular mechanism for the increased stability and nuclear to cytoplasmic transport of μ secretory mRNA has been unexplored. Using a systematic mutational analysis, we have identified three motifs upstream of the μ secretory poly(A) site that inhibit accumulation of poly(A) μ secretory mRNA in vivo and regulate expression of the secretory poly(A) site during B-cell differentiation. All three motifs resemble the U1A-binding site on U1snRNP. It has been shown that U1A, independently of U1snRNP, binds the same sequence in its own 3′-untranslated region (UTR) and regulates its own production by inhibition of addition of a poly(A) tail to its own mRNA (Boelens et al., 1993; Gunderson et al., 1994, 1997). We therefore investigated the possibility that U1A has a similar effect on this heterologous mRNA. We show that recombinant U1A binds the three inhibitory motifs in vitro and that endogenous U1A binds these motifs in conjunction with the cleavage/polyadenylation-specific complex in nuclear extracts. U1A inhibits poly(A) tail addition in vitro and in vivo. U1A inhibits poly(A) addition to the μ secretory mRNA to a lesser extent than it does to its own mRNA, which is more appropriate for regulation of a heterologous mRNA. Furthermore, overexpression of U1A in vivo results in the selective inhibition of the μ secretory mRNA relative to the μ membrane mRNA, demonstrating selective post-cleavage control of the expression of the μ secretory mRNA. We scanned the sequences upstream of the poly(A) sites of the other immunoglobulin isotypes and found evidence that they also use this mechanism. These results are the first demonstration of the physiological importance of the regulation of post-cleavage nuclear poly(A) addition in the regulation of alternative gene expression during development and may be used to regulate alternative expression of other genes, in particular the other immunoglobulin isotypes. Results Identification of multiple sites upstream of the μ secretory poly(A) site that inhibit expression in vivo We have shown previously that the core sequence of the secretory poly(A) site (positions 1951–2085) consists of an extended AU-rich region, consisting of the consensus A2UA3 hexanucleotide sequence and an adjacent upstream AUA5U2A motif that sustains residual activity, and two downstream GU-rich regions (Phillips and Virtanen, 1997; Phillips et al., 1999) (Figure 1B). These sequences contain all the elements necessary and sufficient for cleavage/polyadenylation activity in vivo and to form a specific polyadenylation complex on this poly(A) site in vitro. However, the flanking sequences may be involved in the regulation of poly(A) site expression. To look for sequences involved in the regulation of the accumulation of μ secretory mRNA, we carried out a systematic mutational analysis of the region between the extended AU-rich region and the upstream 5′ splice site (Figure 2; for location of motifs see Figure 1B). For this we used a dual luciferase reporter assay that we have used successfully previously (Phillips et al., 1996, 1999; Phillips and Virtanen, 1997) in which the secretory poly(A) site (1790–2085) is inserted downstream of the firefly luciferase gene in place of the poly(A) site. We systematically scanned the region upstream of the secretory poly(A) site with mutations by replacing eight nucleotides at a time with eight adenosines in sequential order, with three unchanged nucleotides between each sequence of eight, to make a series of 10 separate constructs (see Figure 2B). These constructs (numbered mut1–mut10) all contained a mutated 5′ splice site at position 1810–1815 (GTAAA to CAAAC) in order to exclude the effect of the inhibition by the 5′ splice site on this poly(A) site (Peterson and Perry, 1989; Gunderson et al., 1998). We transfected the constructs in triplicate into HeLa cells and into the plasmacytoma, J558L, along with a reference plasmid expressing Renilla luciferase, and harvested the cells 22 h later. The firefly luciferase activity was corrected for transfection efficiency and the results were expressed as a percentage of the wild-type secretory poly(A) site, and the results for J588L cells are presented in Figure 2A. Figure 2.Identification of motifs upstream of the secretory poly(A) site that inhibit polyadenylation in vivo in a developmentally regulated manner. Luciferase constructs containing the wild-type or mutant secretory poly(A) site from position 1790 to 2085 were transfected in triplicate into J558L, M12.4.1 and WEHI231 cells. (A) Luciferase activity in J558L cells of 10 constructs containing adenosine replacements of eight As in sequential order scanning the 113 nucleotide sequence from position 1838 to 1950 as indicated by bars and numbers in (B). Bars represent the mean of triplicates ± SE in both (A) and (C). (B) The sequence scanned by the adenosine replacements. The individual adenosine replacements are indicated by horizontal bars and the numbers 1–10. The ‘short’ mutations used in (C) are indicated via arrows and are designated 2s, 4s and 8s, respectively. (C) Luciferase activity of constructs containing the ‘short’ mutations 2s, 4s and 8s in combination in J558L, M12.4.1 and WEHI231 cells. (D) A sequence alignment of the consensus U1A-binding motif on U1snRNP with motifs 2, 4 and 8 (as indicated). Download figure Download PowerPoint Mut4 gives the largest release of inhibition, with a 100% increase in luciferase activity, whereas mut8 results in a 50% increase (Figure 2A). Both these mutated sequences include the sequence AUGC (see Figure 2B). Mut2 also results in a small increase in luciferase expression and includes the sequence AGGC. Similar results were obtained in HeLa cells except that mut2 resulted in an increase of 75%, which was greater than that of mut8 in these cells (data not shown). Mut5 and mut10 result in significant decreases in luciferase activity. To confirm that the reduction in luciferase activity of mut5 was not an artifact, we tested several independent isolates of the mut5 plasmid. These produced the same result. Thus regions 5 and 10 upstream of the secretory poly(A) site have a positive effect on expression and will be the subject of a future investigation. We refined the mutational analysis by replacing only three nucleotides in the inhibitory sequences with As. We mutated the sequence motif AUGC that was common to mut4 and mut8 and AGGC that was the closest match in mut 2 (see Figure 2B). We designated these shorter mutations with the suffix ‘s’ for ‘short’. The shorter mutations mut4s and mut8s reproduced the effects of the longer mutations mut4 and mut8 (data not shown). These mutants were combined to produce double mutations and a triple mutation. The triple mutant contains only nine (3 × 3) single nucleotide changes in a 212 nucleotide RNA. To examine whether the inhibitory effect of these sequences on expression of the μ secretory poly(A) site is regulated during B-cell differentiation, we compared the ratio of expression from the luciferase constructs containing the 2s4s8s mutant poly(A) site to the wild type, in cells representing different stages of B-cell differentiation. J558L cells are plasmacytomas that have lost their endogenous immunoglobulin heavy chain and produce only the secretory form of μ mRNA from a transfected IgM heavy chain gene construct, WEHI231 is an immature B-cell line that produces equal quantities of endogenous secretory and membrane μ mRNA, while M12.4.1 cells are intermediate cells that produce endogenous IgG2a and twice as much secretory as membrane μ mRNA from a transfected construct. As can be seen in Figure 2C, the plasmacytomas (J558L) show the least inhibitory effect of the sequences (lowest mutant to wild-type ratio) while the immature B cells (WEHI) show the highest ratio. The luciferase activity from constructs bearing the mutated motifs is similar in all three cells lines; the difference lies in those bearing wild-type sequences (Figure 2C). This shows that these sequences inhibit expression in a developmentally regulated manner. Furthermore, the ratio of the ratio of the J558L cells to the WEHI cells is similar to the ratio previously reported of the half-lives between these two cell lines (Mason et al., 1988). An alignment of the motifs responsible for this inhibition and their flanking sequences revealed that all three sequences bear a resemblance to the U1A-binding site on U1snRNP (see Figure 2D). As U1A, independently of U1snRNP, has been shown to regulate its own production via inhibiting poly(A) addition to its own mRNA, we investigated the possibility that U1A binding of these motifs inhibits addition of a poly(A) tail to this heterologous mRNA. Multiple (AU/GGC) motifs affect U1A binding upstream of the μ secretory poly(A) site We first tested if the inhibitory motifs bind recombinant U1A in vitro. For these binding assays, we used substrates spanning nucleotides 1790–2001 representing the pre-cleaved RNA forms of the poly(A) sequences. Substrate 2001 was used instead of 1998, which is the actual site of cleavage, as inclusion of the additional bases produced cleaner PCR products. Figure 3A shows the in vitro binding pattern as increasing concentrations of recombinant U1A are added to the substrate and the effects of combinations of the short mutations. With the wild-type substrate, three U1A–RNA complexes are discernible, representing the binding of one, two or three U1A proteins, respectively, as the concentration of U1A increases (Figure 3A, lanes 1–8, indicated with arrows). Double mutant combinations of mut2s, mut 4s and mut8s resulted in a significant but not complete loss of U1A binding; the double mutant 2s4s is shown as an example in Figure 3A (lanes 9–16). When all three mutants are combined, U1A binding is abolished (lanes 17–24). Inclusion of mut3s in combinations with mut4s and mut8s had no effect on binding activity, showing that the random introduction of three As alone did not affect binding (data not shown). This analysis demonstrates that U1A binds all three inhibitory motifs in vitro and that each substrate can bind three molecules of U1A simultaneously. We included the pattern of binding of U1A to the inhibitory element in its own 3′-UTR for comparison with that of the μ secretory poly(A) site (Figure 3B). It can be seen that strength of binding of U1A to the μ secretory poly(A) site is lower than that of the binding of U1A to the inhibitory element in its own 3′-UTR. Compare the binding of 10 ng of U1A to the PIE element (Figure 3B, lane 4) with that of 10 ng of U1A to the μ secretory poly(A) site (Figure 3A, lane 4). Figure 3.The mutations that inhibit polyadenylation in vivo affect U1A binding in vitro. Electrophoretic mobility shift assays showing the binding of increasing amounts of recombinant U1A to (A) pre-cleaved immunoglobulin substrates containing either the wild-type sequence or multiple mutations as indicated; and (B) the wild-type or mutated PIE element in the 3′-UTR of U1A mRNA. Download figure Download PowerPoint Endogenous U1A binds the inhibitory motifs in conjunction with the cleavage/polyadenylation-specific complex We next wanted to test whether endogenous U1A binds the inhibitory motifs in vivo and is in a position to affect the function of the cleavage/polyadenylation apparatus. For this, we examined the effect of anti-U1A antibodies on the native cleavage/polyadenylation complex in nuclear extracts formed on the μ secretory poly(A) site in the presence and absence of the inhibitory motifs. The cleavage/polyadenylation-specific complex is identifiable on native gels by its ability to form only on polyadenylation substrates that contain an intact AAU AAA hexanucleotide sequence (Gilmartin and Nevins, 1989) and previously shown for the μ secretory poly(A) site, in particular, by us (Phillips and Virtanen, 1997). We used the same substrates in lanes 1 and 2 of Figure 4A that we used previously to validate the identity of the complex, which is indicated with a bracket. These substrates, extending from position 1951 to 2085 (see Figure 1B), span the sequences we previously have shown to be necessary to bind the core polyadenylation complex (Phillips and Virtanen, 1997). However, they do not contain the inhibitory motifs and are used as a deletion control for the anti-U1A antibody experiments. We compared them with the longer substrates, extending upstream to position 1790, which do span the inhibitory motifs. Figure 4.Endogenous U1A binds RNA in conjunction with the cleavage/polyadenylation-specific complex that forms on the μ secretory poly(A) site in vitro. Electrophoretic mobility shift assays of HeLa cell nuclear extracts. (A) The formation of the polyadenylation-specific complex on immunoglobulin substrates with (lanes 7–10) or without (lanes 1–6) the region spanning the inhibitory motifs. Lanes 1 and 2 contain the substrate IgM(1951–2085) with four U to G mutations in the extended hexanucleotide sequence (Phillips and Virtanen, 1997). (B) The formation of the polyadenylation-specific complex on wild-type (lanes 2–4) or triple mutant (2s4s8s) immunoglobulin substrates (lanes 5–7). Lane 1 contains the substrate IgM(1951–2085) with four U to G mutations in the extended hexanucleotide sequence (Phillips and Virtanen, 1997). Anti-U1A antibodies were added in increasing concentrations as indicated. The positions of the specific and non-specific complexes are indicated. Download figure Download PowerPoint Anti-U1A antiserum 856 did not affect the complex formed on the shorter substrate, IgM(1951–2085) (Figure 4A, lanes 3–6). However, when the substrate is extended upstream to include the inhibitory motif, IgM(1790–2085), introduction of increasing concentrations of anti-U1A antibody disrupts the complex (lanes 7–10). This shows that endogenous U1A binds RNA in conjunction with the native cleavage/polyadenylation-specific complex on the μ secretory poly(A) site when the region containing the U1A-binding sites is included. However, U1A is not part of the specific complex per se formed on the core sequences. Mutation of the inhibitory motifs abolished the ability of anti-U1A antibodies to disrupt the complex in the context of the longer substrate (Figure 4B, compare lanes 5–7 with 2–4). From these results, we conclude that endogenous U1A binds the inhibitory motifs in nuclear extracts and its binding sites are accessible in conjunction with the cleavage/polyadenylation-specific complex. U1A inhibits poly(A) addition at the secretory poly(A) site in vitro As U1A has been shown to inhibit poly(A) addition to its own mRNA (Gunderson et al., 1994), we next investigated whether U1A bound to the novel inhibitory motifs also affects poly(A) addition to this heterologous mRNA in vitro. We measured specific cleavage/polyadenylation specificity factor (CPSF)-dependent poly(A) addition at the μ secretory poly(A) site in nuclear extracts using the same pre-cleaved substrates that were shown to bind recombinant U1A in Figure 3A. Increasing concentrations of recombinant U1A reduced poly(A) addition to the wild-type pre-cleaved substrate but had no effect on the triple mutant pre-cleaved substrate (Figure 5A, lanes 5–9 and 11–15, respectively). This shows that U1A can inhibit poly(A) addition at the μ secretory poly(A) site and that U1A binding to the inhibitory motifs is responsible for this effect. U1A inhibits poly(A) addition to its own mRNA much more strongly than to the μ secretory poly(A) site (Figure 5A, compare lanes 2 and 3 with lanes 5–9). Nevertheless, U1A inhibits poly(A) addition of the μ secretory mRNA by the same mechanism that has been reported previously, namely via an inhibition of poly(A) polymerase (PAP) activity (Gunderson et al., 1994). This is shown in Figure 5B. We performed reconstituted CPSF-independent poly(A) addition assays (Bienroth et al., 1991) using only recombinant PAP, recombinant U1A and either pre-cleaved wild-type or pre-cleaved mut2s4s8s RNAs as substrates (Figure 5B). Increasing concentrations of U1A inhibited PAP activity on the wild-type substrate (lanes 2–6) but had no effect on the triple mutant RNA substrate (lanes 7–12). Thus, U1A bound upstream of the μ secretory poly(A) site has a direct inhibitory effect on PAP similar to its effect in the U1A autoregulatory system (Gunderson et al., 1994). Figure 5.U1A inhibits poly(A) polymerase activity at the secretory poly(A) site. (A) The effect of increasing concentrations of recombinant U1A with specific poly(A) addition in HeLa cell nuclear extracts on wild-type and triple mutant (2s4s8s) substrates. All lanes contain 2 μl of nuclear extracts, total protein content 15 μg, except lanes 1, 4 and 10, which contained no nuclear extract. (B) The effect of increasing concentrations of recombinant U1A with non-specific poly(A) addition on recombinant PAP activity on wild-type and triple mutant (2s4s8s) substrates. Increasing concentrations of recombinant U1A were added as indicated. All lanes contain 50 ng of recombinant PAP, except lanes 1 and 7, which contained no PAP. All substrates (including wild type) contained a mutated 5′ splice site at position 1810–1815. (C) The lengths of poly(A) tails formed in vivo from transfected luciferase constructs containing wild-type or triple mutant (2s4s8s) μ secretory poly(A) sites from Figure 2C (J558L cells) were measured by the LM-PAT method (Salles and Strickland, 1999). Lane 1, a 1 kb ladder (NEN); lane 2, the PCR products, which migrate as smears, for the wild type; lane 3, the products from lane 2 digested with Sau3A; lane 4, the same as lane 2 except that the reverse transcriptase was omitted during the reverse transcription step; lanes 5 and 6, results for the wild type and mutant as indicated. Download figure Download PowerPoint Interestingly, the pattern of poly(A) tail inhibition was different in the two systems. In the recombinant system, increased U1A resulted in shorter poly(A) tails. In contrast, in nuclear extracts, poly(A) tails were not shorter but U1A inhibition resulted in fewer polyadenylated substrates (compare Figure 5B lanes 3–6 with A, lanes 6–9). U1A causes a decreased abundance of normally polyadenylated μ secretory RNA rather than an overall shortening of poly(A) tails in vivo To examine whether the inhibition via the inhibitory motifs resulted in an overall shortening of poly(A) tails or decreased the abundance of normally polyadenylated mRNA tails in vivo, we measured the length of the poly(A) tails formed on the wild-type and 2s4s8s mutated sequences of the μ secretory poly(A) sites placed downstream of the reporter constructs of Figure 2C, J558L cells. For this, we used a LM-PAT (ligation method, poly(A) tail measurement) assay (Salles and Strickland, 1999). We purified poly(A)+ mRNA from 107 transfected cells, which were found to have equivalent transfection efficiency as measured by Renilla activity. To amplify specifically the polyadenylated μ secretory sequences, we used two primers: (i) an oligo(dT) ‘anchor’ that anneals to poly(A) and (ii) a primer that hybridizes specifically with the μ secretory sequences present (positions 1790–1807). The primers were designed to span a Sau3A site at position 1838, which allowed verification of the PCR product by enzyme digestion (see Figure 5C, lane 3). We included a control with no reverse transcriptase to verify that genomic DNA was not amplified (Figure 5C, lane 4). The PCR products appear as smears that correspond to poly(A) tail length (see Figure 5C). Figure 5C shows that mRNA from the wild-type and the triple mutant constructs form poly(A) tails of the same length (Figure 5C, compare lanes 5 and 6). The peak of intensity for both smears appears at ∼400, consistent with poly(A) tails of 200 residues for both, although the PCR product smear from the triple mutant appears more spread out in both directions than that from the wild type. The triple mutant is more intense than the wild type, consistent with an increased abundance of the poly(A)+ mutant mRNA. We conclude that poly(A) tails, if formed on the respective mRNAs, do not differ in ultimate length. Rather, these results are consistent with a reduction in the abundance of normally polyadenylated μ secretory sequences as a result of U1A inhibition. The U1A-binding motifs inhibit accumulation of polyadenylated μ secretory mRNA in vivo We next directly determined whether U1A decreases the abundance of normally polyadenylated μ secretory mRNA in vivo, in the context of the μ heavy chain gene, using quantitative RNase protection analysis (Figure 6). For this we used M12.4.1 cells, which produce a detectable amount of μ membrane mRNA from the transfected μ heavy chain-expressing plasmid, pμΔ3 (Grosschedl and Baltimore, 1985), providing a convenient internal reference for measurement of the accumulation of the secretory form of μ mRNA. These cells endogenously express IgG2a (Glimcher et al., 1982), which does not hybridize with the μ antisense probe used in the RNase protection assays (Figure 6A, lane 4). Figure 6.U1A-binding motifs inhibit accumulation of polyadenylated μ secretory mRNA in vivo. RNase protection assays. Wild-type or mutant plasmids containing the IgM heavy chain gene were transfected into M12.4.2 cells in triplicate. (A) RNase protection assay visualized by phosphorimagery. Lane 1 is the 32P-radiolabeled pBR322 MspI digest marker. Lanes 2–4 are mock, wild-type and mutant transfected poly(A)-selected mRNA, respectively. The secretory and membrane-protected fragments are indicated with brackets. (B) Phosphorimagery analysis of (A). The secretory measurements were corrected for the higher level of [32P]UTP due to a longer protected fragment and all the results were expressed as a ratio of the wild-type membrane-protected fragment. Bars represent the mean of triplicates ± SE. Download figure Download PowerPoint At 17 h after transfection of wild-type- or triple mutant-containing pμΔ3 plasmids, poly(A)+ mRNA was extracted and subjected to RNase protection analysis using wild-type or mutant antisense RNA spanning the 5′ splice site and the secretory poly(A) site (see Figure 1C for location of the antisense probe and expected protected fragments). The expected sizes of the protected secretory and membrane mRNA fragments are 268 and 80 nucleotides, respectively. Triplicate analyses were quantitated and a representative of each of the wild type and mutant are presented in Figure 6A. As can be seen in Figure 6A, introduction of mutations into the U1A-binding sites in the context of the whole μ heavy chain gene substantially increases the amount of polyadenylated μ secretory mRNA (compare the mutant with the wild type in lanes 3 and 2, respectively). The phosphorimagery values for the secretory product were corrected for the higher level of [32P]UTP due to a longer protected fragment, and all results were expressed as a ratio of the wild-type membrane protected fragment (see Figure 6B). For the wild type, the ratio of the secretory to the membrane form of mRNA was 1.9 ± 0.2 SE, which is comparable with what has been reported previously for this cell line (Peterson et al., 1991). Upon introduction of the mutations in the U1A-binding sites, this ratio increases to 4.0 ± 0.2. Thus, the U1A-binding motifs upstream of the secretory poly(A) site significantly inhibit the accumulation of polyadenylated μ secretory mRNA. Overexpression of U1A in vivo selectively inhibits the secretory form of mRNA We tested the effect of overexpression of U1A on expression of the μ secretory form in vivo in M12.4.1 cells. We transfected sets of plasmids containing U1A in which the polyadenylation inhibitory element (P
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