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Cross-Kingdom Amplification Using Bacteria -Specific Primers: Complications for Studies of Coral Microbial Ecology

2008; American Society for Microbiology; Volume: 74; Issue: 24 Linguagem: Inglês

10.1128/aem.01303-08

1098-5336

Julia P. Galkiewicz, Christina A. Kellogg,

Genomics and Phylogenetic Studies

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.