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Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture

2013; Multidisciplinary Digital Publishing Institute; Volume: 18; Issue: 3 Linguagem: Inglês

10.3390/molecules18032528

ISSN

1433-1373

Autores

Gezimar D. de Souza, Axel Mithöfer, Cristina Daolio, Bernd Schneider, Edson Rodrigues‐Filho,

Tópico(s)

Plant Pathogens and Fungal Diseases

Resumo

This present work describes the application of liquid chromatography-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC(50) values which range from 0.11 (± 0.02) to 4.69 (± 0.47) μM showed the high cytotoxicity of these compounds.

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