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Real‐time mid‐infrared imaging of living microorganisms

2015; Wiley; Volume: 9; Issue: 1-2 Linguagem: Inglês

10.1002/jbio.201500264

1864-0648

Katharina Haase, Niels Kröger‐Lui, Annemarie Pucci, Arthur Schönhals, Wolfgang Petrich,

Advanced Fluorescence Microscopy Techniques

The speed and efficiency of quantum cascade laser-based mid-infrared microspectroscopy are demonstrated using two different model organisms as examples. For the slowly moving Amoeba proteus, a quantum cascade laser is tuned over the wavelength range of 7.6 µm to 8.6 µm (wavenumbers 1320 cm(-1) and 1160 cm(-1) , respectively). The recording of a hyperspectral image takes 11.3 s whereby an average signal-to-noise ratio of 29 is achieved. The limits of time resolution are tested by imaging the fast moving Caenorhabditis elegans at a discrete wavenumber of 1265 cm(-1) . Mid-infrared imaging is performed with the 640 × 480 pixel video graphics array (VGA) standard and at a full-frame time resolution of 0.02 s (i.e. well above the most common frame rate standards). An average signal-to-noise ratio of 16 is obtained. To the best of our knowledge, these findings constitute the first mid-infrared imaging of living organisms at VGA standard and video frame rate.