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Anthracnose on Strawberry Fruits Caused by Colletotrichum siamense in Brazil

2015; American Phytopathological Society; Volume: 100; Issue: 4 Linguagem: Inglês

10.1094/pdis-10-15-1121-pdn

1943-7692

Nayara Pereira Capobiango, Danilo Batista Pinho, Laércio Zambolim, Olinto Liparini Pereira, Ueder Pedro Lopes,

Banana Cultivation and Research

HomePlant DiseaseVol. 100, No. 4Anthracnose on Strawberry Fruits Caused by Colletotrichum siamense in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseAnthracnose on Strawberry Fruits Caused by Colletotrichum siamense in BrazilN. P. Capobiango, D. B. Pinho, L. Zambolim, O. L. Pereira, and U. P. LopesN. P. Capobiango, D. B. Pinho, L. Zambolim, O. L. Pereira, and U. P. LopesAffiliationsAuthors and Affiliations N. P. Capobiango , Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil D. B. Pinho , Departamento de Fitopatolologia, Universidade de Brasília, Brasília, Distrito Federal, 70.910-900, Brazil L. Zambolim O. L. Pereira , Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil U. P. Lopes , Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, Pernambuco, 55.292-270, Brazil. Published Online:5 Feb 2016https://doi.org/10.1094/PDIS-10-15-1121-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Anthracnose of strawberries (Fragaria × ananassa), caused by Colletotrichum spp., has been mainly associated with the C. acutatum complex (Baroncelli et al. 2015; Damm et al. 2012). This disease is considered one of the main diseases in strawberry fields in Brazil. In June 2012, an isolate typical of the C. gloeosporioides complex was obtained from fruits located in the municipality of Viçosa, Minas Gerais, Brazil. Symptoms initially appeared as circular, sunken necrotic lesions; under humid conditions, pink spore masses were observed on the lesion. This disease affected approximately 30% of the fruits in the field and postharvest. A monosporic culture obtained on potato dextrose agar (PDA) medium was deposited at the Coleção de Culturas de Fungos Fitopatogênicos "Prof. Maria Menezes" (code CMM 3987). The characteristics of the conidia (hyaline, aseptate, fusiform with obtuse ends, smooth-walled, guttulate, 13 to 18 µm long and 4.5 to 8 µm wide) matched the morphological characteristics of the C. gloeosporioides complex. For accurate identification, partial sequences of actin (ACT), chitin synthase (CHS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3), internal transcribed spacer (ITS), manganese-superoxide dismutase (SOD2), and β-tubulin 2 (β-TUB) were obtained and deposited in GenBank (Accession Nos. KT693099 to KT693105), and a search within the Q-bank Fungi database retrieved C. siamense with a high percentage of identity (>99%). Additionally, Bayesian inference analysis using concatenated sequences (ACT, CHS, GAPDH, ITS, SOD2, and β-TUB) placed our isolate in the same clade with other C. siamense samples (study S18199 deposited in TreeBASE). To verify pathogenicity, Koch's postulates were performed. Ten strawberry fruits cv. Camarosa were inoculated with a drop (5 μl) of conidial suspension (106 conidia/ml), while a drop (5 μl) of distilled water was deposited on control fruits. After inoculation, the fruits were placed in a tray, covered with a transparent plastic bag, and incubated at 25°C in the dark. Fruit rot symptoms were observed on inoculated fruits three days after inoculation. The control fruits remained healthy. The fungus was reisolated from the lesion and identified as C. siamense using the methods above. In the C. gloeosporioides species complex, only C. aenigma, C. fructicola, C. siamense, and C. theobromicola were previously reported on strawberries (Weir et al. 2012; Baroncelli et al. 2015). Although C. siamense has a wide host range, this pathogen was reported only on coffee in Brazil (Silva et al. 2012). To our knowledge, this is the first report of C. siamense causing fruit rot (anthracnose) on strawberries in Brazil.References:Baroncelli, R., et al. 2015. PLoS One 10:e0129140. https://doi.org/10.1371/journal.pone.0129140 Crossref, ISI, Google ScholarDamm, U., et al. 2012. Stud. Mycol. 73:37. https://doi.org/10.3114/sim0010 Crossref, ISI, Google ScholarSilva, D. N., et al. 2012. Mycologia 104:396. https://doi.org/10.3852/11-145 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 4 April 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 23 Mar 2016Published: 5 Feb 2016First Look: 23 Nov 2015Accepted: 7 Nov 2015 Pages: 859-859 Information© 2016 The American Phytopathological SocietyCited byTwo additional Colletotrichum species causing leaf spot of rambutan ( Nephelium lappaceum )16 March 2023 | Archives of Phytopathology and Plant Protection, Vol. 102Colletotrichum species pathogenic to strawberry: discovery history, global diversity, prevalence in China, and the host range of top two species16 November 2022 | Phytopathology Research, Vol. 4, No. 1Colletotrichum siamenseCABI Compendium, Vol. CABI CompendiumFirst report of anthracnose on Spigelia anthelmia caused by Colletotrichum karstii and Colletotrichum siamense in Brazil11 March 2021 | Journal of Plant Diseases and Protection, Vol. 128, No. 3Characterization of fungal species associated with cladode brown spot on Nopalea cochenillifera in Brazil11 September 2019 | European Journal of Plant Pathology, Vol. 155, No. 4First Report of Anthracnose Causing Both Crown and Fruit Rot of Strawberry by Colletotrichum siamense in North CarolinaT. 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