Produção Nacional
Revisado por pares
First Report of Tomato chlorosis virus Infecting Eggplant and Scarlet Eggplant in Brazil
2015; American Phytopathological Society; Volume: 100; Issue: 4 Linguagem: Inglês
10.1094/pdis-09-15-1087-pdn
ISSN1943-7692
AutoresM. E. N. Fonseca, L. S. Boiteux, M. F. Lima, J. L. de Mendonça, Alexandre Florian da Costa, M. G. Fontes, Hélcio Costa, M. González-Arcos,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 100, No. 4First Report of Tomato chlorosis virus Infecting Eggplant and Scarlet Eggplant in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato chlorosis virus Infecting Eggplant and Scarlet Eggplant in BrazilM. E. N. Fonseca, L. S. Boiteux, M. F. Lima, J. L. Mendonça, A. F. Costa, M. G. Fontes, H. Costa, and M. González-ArcosM. E. N. Fonseca, L. S. Boiteux, M. F. Lima, J. L. Mendonça, A. F. Costa, M. G. Fontes, H. Costa, and M. González-ArcosAffiliationsAuthors and Affiliations M. E. N. Fonseca L. S. Boiteux M. F. Lima J. L. Mendonça A. F. Costa , CNPH-Embrapa, Brasília-DF, Brazil M. G. Fontes , Dept. Fitopatologia, UnB, Brasília-DF, Brazil H. Costa , Incaper, Venda Nova do Imigrante-ES, Brazil M. González-Arcos , INIA, Salto Grande, Uruguay. Published Online:17 Feb 2016https://doi.org/10.1094/PDIS-09-15-1087-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Eggplant (Solanum melongena L.) and scarlet eggplant (S. aethiopicum L.) are economically important vegetable crops in warm regions of Brazil. Samples of the eggplant cultivars 'Napoli' and 'Ciça' with interveinal yellowing in the older leaves similar to that induced by criniviruses were collected in fields heavily colonized by Bemisia tabaci MEAM-1 species in Canguçu-Rio Grande do Sul State in February 2013 (isolate named as CR-131) and Formosa-Goiás State in April 2015 (isolate CR-173). Scarlet eggplants 'Comprido' showing identical symptoms were also sampled in Venda Nova do Imigrante–Espírito Santo State in April 2013 (isolate CR-148), Bragança Paulista-São Paulo State in June 2013 (isolate CR-155), and Brasília–Federal District in June 2015 (isolate CR-193). In order to check for infection by the criniviruses Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV), RT-PCR assays were carried out (with one sample per site) using the primer pair HS-11/HS-12. All symptomatic samples displayed a 587-bp amplicon, corresponding to the HSP-70 gene homolog, which is highly conserved across crinivirus genomes. The RT-PCR products were used as templates in nested PCR assays with ToCV (ToC-5/ToC-6) and TICV-specific (TIC-3/TIC-4) primers (Dovas et al. 2002). Only ToCV-specific amplicons (463 bp) were obtained in all five isolates, indicating the ToCV presence, but not TICV. Amplicons of the two eggplant (GenBank Accession Nos. KT727949 and KT727950) and the three scarlet eggplant isolates (KT727954 to KT727956) were sequenced. Alignments showed 100% nucleotide identity with one another and 99.7% identity with one tomato ToCV isolate from Brazil (Accession No. EU868927). Dot blot hybridization assays with a ToCV coat protein gene-derived RNA probe also confirmed the ToCV infection in all samples. Transmission tests from ToCV-infected eggplant and scarlet eggplant to seedlings of the begomovirus-resistant tomato line 'TX 468 RG' (Giordano et al. 2005) were positive using viruliferous B. tabaci MEAM-1 adults. Inoculated plants displayed interveinal chlorosis around 60 days after inoculation. In South America, ToCV has been detected so far on solanaceous crops and weeds (Fonseca et al. 2013; Arruabarrena et al. 2015). However, this is the first formal report of S. melongena and S. aethiopicum as natural hosts of ToCV in the continent and the first worldwide report of scarlet eggplant as a crinivirus host. The wide geographical distribution of these new ToCV hosts in Brazil may have direct epidemiological implications since they are very often cultivated side-by-side with tomatoes. Therefore, they may serve as sources of inoculum to the tomato crop in which ToCV infection has become a major problem throughout many producing regions in the Southern Cone of South America (Arruabarrena et al. 2014).References:Arruabarrena, A., et al. 2014. Plant Dis. 98:1445. https://doi.org/10.1094/PDIS-11-13-1153-PDN Link, ISI, Google ScholarArruabarrena, A., et al. 2015. Plant Dis. 99:895. https://doi.org/10.1094/PDIS-12-14-1315-PDN Link, ISI, Google ScholarDovas, C. I., et al. 2002. Plant Dis. 86:1345. https://doi.org/10.1094/PDIS.2002.86.12.1345 Link, ISI, Google ScholarFonseca, M. E. N., et al. 2013. Plant Dis. 97:692. https://doi.org/10.1094/PDIS-06-12-0593-PDN Link, ISI, Google ScholarGiordano, L. B., et al. 2005. Euphytica 143:27. https://doi.org/10.1007/s10681-005-1685-1 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 4 April 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 23 Mar 2016Published: 17 Feb 2016First Look: 23 Nov 2015Accepted: 16 Nov 2015 Page: 867 Information© 2016 The American Phytopathological SocietyCited byTomato chlorosis virus, a promiscuous virus with multiple host plants and whitefly vectors2 November 2022 | Annals of Applied Biology, Vol. 182, No. 1Novel natural hosts of tomato severe rugose virus (ToSRV) in the Fabaceae, Solanaceae, and Oxalidaceae families19 October 2021 | Journal of Plant Diseases and Protection, Vol. 129, No. 2Bemisia tabaci (tobacco whitefly)CABI Compendium, Vol. CABI CompendiumTomato chlorosis virus (yellow leaf disorder of tomato)CABI Compendium, Vol. CABI CompendiumNatural Infection Rate of Known Tomato chlorosis virus-Susceptible Hosts and the Influence of the Host Plant on the Virus Relationship With Bemisia tabaci MEAM1Arnaldo Esquivel-Fariña, Jorge Alberto Marques Rezende, William M. 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Fonseca15 August 2019 | Plant Disease, Vol. 103, No. 10Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges2 July 2019 | Molecular Plant Pathology, Vol. 20, No. 9Expanding Knowledge of the Host Range of Tomato chlorosis virus and Host Plant Preference of Bemisia tabaci MEAM1Arnaldo E. Fariña, Jorge A. M. Rezende, and William M. Wintermantel17 April 2019 | Plant Disease, Vol. 103, No. 6Biochemical, Serological, Molecular and Natural Host Studies on Tomato Chlorosis Virus in EgyptPakistan Journal of Biological Sciences, Vol. 22, No. 2Solanum aethiopicum (African eggplant / Scarlet eggplant)6 June 2020Solanum melongena (Brinjal, Eggplant, Aubergine)6 June 2020Performance of Bemisia tabaciMEAM 1 and Trialeurodes vaporariorum on Tomato chlorosis virus (To CV ) infected plants16 August 2018 | Journal of Applied Entomology, Vol. 142, No. 10Identification of genetic sources with attenuated Tomato chlorosis virus-induced symptoms in Solanum (section Lycopersicon) germplasm22 September 2018 | Euphytica, Vol. 214, No. 10Identification of Eight Solanum (subgenus Leptostemonum) Species as Novel Natural Hosts of Tomato chlorosis virus in BrazilL. S. Boiteux, M. F. Lima, M. E. N. Fonseca, J. L. Mendonça, A. F. Costa, J. G. Silva-Filho, M. G. Fontes, and M. González-Arcos24 May 2018 | Plant Disease, Vol. 102, No. 8
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