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First Report of Anthracnose on Arctium lappa Caused by Colletotrichum orchidearum in China

2015; American Phytopathological Society; Volume: 100; Issue: 5 Linguagem: Inglês

10.1094/pdis-08-15-0945-pdn

1943-7692

XU Hai-tao, Rujun Zhou, J. F. Fu, Jiamin Zhao, liru liu,

Yeasts and Rust Fungi Studies

HomePlant DiseaseVol. 100, No. 5First Report of Anthracnose on Arctium lappa Caused by Colletotrichum orchidearum in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Anthracnose on Arctium lappa Caused by Colletotrichum orchidearum in ChinaH. J. Xu, R. J. Zhou, J. F. Fu, J. F. Zhao, and L. LiuH. J. XuSearch for more papers by this author, R. J. ZhouSearch for more papers by this author, J. F. FuSearch for more papers by this author, J. F. ZhaoSearch for more papers by this author, and L. LiuSearch for more papers by this authorAffiliationsAuthors and Affiliations H. J. Xu R. J. Zhou J. F. Fu J. F. Zhao L. Liu , Department of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, 110866, China. Published Online:25 Feb 2016https://doi.org/10.1094/PDIS-08-15-0945-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Arctium lappa (family Compositae) is a perennial herb highly valued as a medicinal herb and vegetable in China and Japan. In July 2014, symptoms typical of anthracnose were observed on leaves of A. lappa in a production field in Bingyugou, Liaoning, China. The disease mainly affected leaves, with symptoms on ∼10 to 20% of plants. Yield was reduced ∼5 to 8%. Symptoms initially appeared as small, slightly sunken, circular, water-soaked spots, which enlarged and developed into numerous brown, scattered, irregular lesions (4 to 12 mm in diameter), surrounded by a yellow ring. Later, lesions became cracked at the centers of the spots and coalesced. Acervuli were usually epiphyllous, globose or oblate, 125.5 to 188.24 × 58.4 to 102.1 μm (avg. 154.1 × 70.5 μm) on the lesions under moist conditions. Setae were nonbranching, pale to dark brown, smooth-walled or verruculose, 70.2 to 174.6 μm long, with 3 to 6-septa. Conidia were unicellular, aseptate, hyaline, straight, and cylindrical, with both apices rounded, and 13.4 to 19.7 × 3.5 to 7.7 μm (average 16.7 × 5.8 μm). The pathogen was isolated from symptomatic tissues, and cultured on PDA at 25°C (Li et al. 2013). Three representative isolates were selected for further studies, in which isolate SAUCC 1407 was designated the holotype and deposited in the Herbarium of the Shenyang Agricultural University. Colonies initially produced white aerial mycelium and later became buff to orange with an average growth rate of 12.8 mm/day. After 15 days, dark brown, subglobose acervuli interspersed within the colonies. The ITS, beta-tubulin (TUB2), calmodulin (CAL), chitin synthase 1 (CHS-1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and actin (ACT) region of rDNA was amplified with primers ITS1/ITS4, Bt2a/Bt2b, CL1/CL2, CHS-354R/CHS-79F, GDF1/GDR1, and ACT-512F/ACT-783R, respectively, and sequenced (Cannon et al. 2012). A phylogenetic tree was produced using MEGA 4.0, and Neighbor-Joining (NJ) analyses were performed using the combined datasets of the 6 regions, forming a single clade with maximum support with Colletotrichum orchidearum (Yang et al. 2011). Representative sequences of the ITS (561 bp), TUB (485 bp), CAL (725 bp), CHS-1 (298 bp), GAPDH (230 bp), and ACT (242 bp) were deposited in GenBank as KT362184 to KT362189, respectively. Thus, the fungus was identified as C. orchidearum on the basis of morphological characteristics and multilocus phylogenetic analysis. To confirm pathogenicity, five healthy, detached leaves of A. lappa were inoculated with 106 conidia/ml of each representative isolate, while another 5 control leaves were atomized with sterilized water. The leaves were kept in a sealed plastic container layered with moist filter paper at 25°C in natural daylight. Typical symptoms of anthracnose on inoculated leaves were reproduced after 8 to 10 days; control leaves remained healthy. The same fungus was reisolated from the symptomatic tissue and identified as C. orchidearum based on the morphological characters, completing Koch’s postulates. To our knowledge, this is the first report of anthracnose on A. lappa caused by C. orchidearum in China and may be useful in the establishment of a base for scientific control of the disease.References:Cannon, P. F., et al. 2012. Stud. Mycol. 73:181. https://doi.org/10.3114/sim0014 Crossref, ISI, Google ScholarLi, Y., et al. 2013. Eur. J. Plant Pathol. 137:127. https://doi.org/10.1007/s10658-013-0224-5 Crossref, ISI, Google ScholarYang, Y. L., et al. 2011. Cryptogam., Mycol. 32:229. https://doi.org/10.7872/crym.v32.iss3.2011.229 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 5 May 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Apr 2016Published: 25 Feb 2016First Look: 23 Nov 2015Accepted: 17 Nov 2015 Pages: 1010-1010 Information© 2016 The American Phytopathological SocietyCited byDiseases of Burdock31 January 2023Characterization and Pathogenicity of Colletotrichum Species on Philodendron tatei cv. Congo in Gansu Province, ChinaLonghai Xue, Yongwen Zhang, Tingyu Duan, Mengyuan Li, James F. White, Yong Liu, and Chunjie Li20 August 2020 | Plant Disease, Vol. 104, No. 10The Colletotrichum dracaenophilum , C. magnum and C. orchidearum species complexesStudies in Mycology, Vol. 92, No. 1