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Zooshikella marina sp. nov. a cycloprodigiosin- and prodigiosin-producing marine bacterium isolated from beach sand

2015; Microbiology Society; Volume: 65; Issue: Pt_12 Linguagem: Inglês

10.1099/ijsem.0.000630

1466-5034

E. V. V. Ramaprasad, Dave Bharti, Ch. Sasikala, Ch. V. Ramana,

Microbial Natural Products and Biosynthesis

A red-pigmented bacterium producing a metallic green sheen, designated strain JC333 T , was isolated from a sand sample collected from Shivrajpur–Kachigad beach, Gujarat, India. Phylogenetic analyses based on the 16S rRNA gene sequence of strain JC333 T showed highest sequence similarity to Zooshikella ganghwensis JC2044 T (99.24 %) and less than 91.94 % similarity with other members of the class Gammaproteobacteria . DNA–DNA hybridizations between JC333 T and Z. ganghwensis JC2044 T showed low relatedness values of 19 ± 1.3 % (reciprocal 21 ± 2.2 %). The major respiratory quinone was ubiquinone-9 (Q9) and the polar lipid profile was composed of the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. The presence of C 16 : 1 ω7 c /C 16 : 1 ω6 c , C 16 : 0 , C 18 : 1 ω7 c and C 12 : 0 as major fatty acids supported the affiliation of strain JC333 T to the genus Zooshikella . Prodigiosin, cycloprodigiosin and eight other prodigiosin analogues were the pigments of JC333 T . Characterization based on 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, ubiquinone, and polar lipid and fatty acid compositions revealed that JC333 T represents a novel species of the genus Zooshikella , for which the name Zooshikella marina sp. nov. is proposed. The type strain is JC333 T ( = KCTC 42659 T = LMG 28823 T ).