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Principles of connectivity among morphologically defined cell types in adult neocortex

2015; American Association for the Advancement of Science; Volume: 350; Issue: 6264 Linguagem: Inglês

10.1126/science.aac9462

1095-9203

Xiaolong Jiang, Shan Shen, Cathryn R. Cadwell, Philipp Berens, Fabian H. Sinz, Alexander S. Ecker, Saumil S. Patel, Andreas S. Tolias,

Neural dynamics and brain function

Since the work of Ramón y Cajal in the late 19th and early 20th centuries, neuroscientists have speculated that a complete understanding of neuronal cell types and their connections is key to explaining complex brain functions. However, a complete census of the constituent cell types and their wiring diagram in mature neocortex remains elusive. By combining octuple whole-cell recordings with an optimized avidin-biotin-peroxidase staining technique, we carried out a morphological and electrophysiological census of neuronal types in layers 1, 2/3, and 5 of mature neocortex and mapped the connectivity between more than 11,000 pairs of identified neurons. We categorized 15 types of interneurons, and each exhibited a characteristic pattern of connectivity with other interneuron types and pyramidal cells. The essential connectivity structure of the neocortical microcircuit could be captured by only a few connectivity motifs.