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First Report of Root Rot and Vine Decline of Melon Caused by Monosporascus cannonballus in Eastern Mainland China

2015; American Phytopathological Society; Volume: 100; Issue: 3 Linguagem: Inglês

10.1094/pdis-06-15-0655-pdn

1943-7692

Yan Li, Qiao-Lu Zang, Yunping Huang, Y. H. Wang,

Plant Disease Resistance and Genetics

HomePlant DiseaseVol. 100, No. 3First Report of Root Rot and Vine Decline of Melon Caused by Monosporascus cannonballus in Eastern Mainland China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Root Rot and Vine Decline of Melon Caused by Monosporascus cannonballus in Eastern Mainland ChinaL. Y. Yan, Q. Y. Zang, Y. P. Huang, and Y. H. WangL. Y. Yan, Q. Y. Zang, Y. P. Huang, and Y. H. WangAffiliationsAuthors and Affiliations L. Y. Yan Q. Y. Zang Y. P. Huang Y. H. Wang , Institute of Vegetables and the Key Lab of Cucurbitaceous Vegetables Breeding in Ningbo City, Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China. Published Online:25 Jan 2016https://doi.org/10.1094/PDIS-06-15-0655-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In September 2014, a root rot and vine decline was observed on melon (Cucumis melo L.) growing in several greenhouses with 85% disease incidence in Zhejiang Province in eastern mainland China. This pathogen had been previously separated from melon in a small-area test field in Gansu Province in mainland China (He and Bai 2010). The diseased melons exhibited chlorosis of crown leaves and collapse of vines just prior to harvest. The roots of affected plants exhibited necrotic lesions and loss of secondary and tertiary roots. Brown discolorations of vascular tissues was apparent but rarely extended more than a few centimeters in aboveground plant parts. Numerous perithecia in the root cortex were visible to the naked eye several days after plant death. Small pieces of symptomatic roots were surface sterilized for 1 min with 1% sodium hypochlorite, rinsed in sterile water, and then placed on potato dextrose agar (PDA) medium amended with streptomycin sulfate (25 mg/liter). A pale brown colony with white aerial mycelia developed after incubation at 25°C in the dark for 7 days. Black, spherical perithecia, 400 μm in diameter, were present 20 days after incubation on PDA. Each ascus contained a single spherical ascospore, 26 to 30 μm in diameter, typical of Monosporascus cannonballus Pollack & Uecker (Pollack and Uecker 1974). DNA from this isolate was extracted and the internal transcribed spacer (ITS) region was amplified using the primers ITS1 and ITS4 (White et al. 1990), sequenced, and submitted to GenBank (Accession No. KT826570). BLAST indicated that the 564-bp sequence shared 99% identity with M. cannonballus in C. melo (Accession No. AM167937.1). Pathogenicity tests were performed on melon (cv. Yellow 9818) in a temperature-controlled growth chamber. Five 7-day-old mycelial plugs (5 mm in diameter) from colony periphery were inoculated to 100 ml sand-wheat grain medium (boiled bulgur wheat grain and sand at 3:1 by volume) and kept at 25°C in the dark for 15 days. The medium overgrown with mycelial was used as inoculum. Five melon seeds were sown in sterilized potting mixture (equal parts sand and peat moss by volume) and inoculum was added to reach a soil concentration of 5%. Five melons grown in noninoculated containers were used as controls. The plants inoculated showed root necrosis and cotyledon wilt after 10 days with a 12-h photoperiod at 30 to 20°C while the controls were symptomless. Monosporascus cannonballus was reisolated from symptomatic roots confirming Koch's postulates. This disease has been reported on watermelon and/or melon in 19 countries around the world, including Egypt, India, Italy, Japan, Korea, Mexico, Spain, Taiwan, and the United States (Cohen et al. 2012; Martyn and Miller 1996). To our knowledge, this is the first report of M. cannonballus on melon in eastern mainland China, extending the known range of the pathogen in China. Due to the wide distribution of melon cultivation and the loose transport policy to plant materials, there is a potential risk for disease spread throughout the country. Grafting of melon onto Cucurbita spp. more tolerant to the pathogen would be an effective disease management.References:Cohen, R., et al. 2012. Hortic. Rev. (Am. Soc. Hortic. Sci.) 39:77. Google ScholarHe, S. Q., and Bai, B. 2010. Plant Pro. 36:116 (In Chinese). Google ScholarMartyn, R. D., and Miller, M. E. 1996. Plant Dis. 80:716. https://doi.org/10.1094/PD-80-0716 Crossref, ISI, Google ScholarPollack, F. G., and Uecker, F. A. 1974. Mycol. 66:346. https://doi.org/10.2307/3758370 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis, eds. Academic Press, San Diego, CA. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 3 March 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 2 Mar 2016Published: 25 Jan 2016First Look: 27 Oct 2015Accepted: 16 Oct 2015 Pages: 651-651 Information© 2016 The American Phytopathological SocietyCited byMonosporascus cannonballusCABI Compendium, Vol. CABI CompendiumCharacterization of Five New Monosporascus Species: Adaptation to Environmental Factors, Pathogenicity to Cucurbits and Sensitivity to Fungicides10 September 2020 | Journal of Fungi, Vol. 6, No. 3Resistance in melon to Monosporascus cannonballus and M. eutypoides : Fungal pathogens associated with Monosporascus root rot and vine decline30 April 2020 | Annals of Applied Biology, Vol. 177, No. 1Prevalent weeds collected from cucurbit fields in Northeastern Brazil reveal new species diversity in the genus Monosporascus19 February 2019 | Annals of Applied Biology, Vol. 174, No. 3REACTION OF MELON GENOTYPES TO THE ROOT´S ROT CAUSED BY Monosporascus1 March 2019 | Revista Caatinga, Vol. 32, No. 1Cotton, cowpea and sesame are alternative crops to cucurbits in soils naturally infested with Monosporascus cannonballus6 March 2018 | Journal of Phytopathology, Vol. 166, No. 6