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MONITORING OF SKIN PENETRATION AND ABSORPTION WITH A NEW IN VIVO EXPERIMENTAL MODEL

2014; Volume: 62; Issue: 6 Linguagem: Romeno

2065-0019

Szilvia Berkó, Boglárka Balázs, Blanka Sütő, Gábor Erős, Brigitta Gál, Anita Sztojkov‐Ivanov, Codruța Şoica, Piroska Szabó‐Révész, Lajos Kemény, István Zupkó, Erzsébet Csányi,

Contact Dermatitis and Allergies

The purpose of the study was to use a new type of in vivo animal model developed by our research group for the investigation of skin penetration, compared to the results of Franz cell measurements. The new in vivo experimental model provides possibility for the exact measurement of the quantity of the penetrated drug through the living mouse skin. Two gel formulations containing ibuprofen were investigated with or without penetration enhancer. The applied penetration enhancer was Transcutol ® . The effect of the penetration enhancer was clearly detected in both models. The in vitro diffused amount of ibuprofen was significantly higher than the one permeated through the living skin. The presence of the active agent in the plasma was also determined after the observation period. It was possible to monitor the penetration and absorption of topical formulations simultaneously. Rezumat Obiectivul studiului a fost utilizarea unui nou tip de model animal in vivo realizat de grupul nostru de cercetare in scopul investigarii penetrarii prin piele, in comparaţie cu rezultatele masuratorilor prin celula Franz. Noul model experimental in vivo ofera posibilitatea unei masurari exacte a cantitaţii de substanţa medicamentoasa care penetreaza prin pielea de soarece. Au fost investigate doua formulari de tip gel cu ibuprofen, cu sau fara promotor de absorbţie. Promotorul de absorbţie utilizat a fost Transcutol ® . Efectul promotorului de absorbţie a fost clar detectat in ambele modele. Cantitatea de ibuprofen difuzata in vitro a fost semnificativ mai mare decât cea difuzata prin pielea de soarece. Prezenţa agentului terapeutic in plasma a fost de asemenea determinata dupa perioada de observaţie. A fost posibila monitorizarea simultana a penetrarii si absorbţiei ambelor formulari topice.