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Inhibition of Elastin Synthesis by High Potassium Salt Is Mediated by Ca2+ Influx in Cultured Smooth Muscle Cells In Vitro: Reciprocal Effects of K+ on Elastin and Collagen Synthesis

1994; Oxford University Press; Volume: 115; Issue: 3 Linguagem: Inglês

10.1093/oxfordjournals.jbchem.a124371

1756-2651

Ichiro Tokimitsu, Shingo Tajima,

Hydrogen embrittlement and corrosion behaviors in metals

Elastin synthesis in cultured smooth muscle cells was inhibited by one fourth in the presence of 0.1 M K+ in the medium. The degree of inhibition paralleled the decrease in the steady-state levels of elastin mRNA. The inhibition of elastin synthesis was blocked by addition of 1 microM nifedipine, a Ca2+ antagonist. Comparable inhibition of elastin synthesis was observed by addition of A23187, a Ca2+ ionophore. In contrast, collagen synthesis and thymidine uptake were stimulated threefold and twofold respectively in the presence of 0.1 M K+ with a concomitant increase in collagen mRNA. The stimulation of collagen synthesis was also blocked by nifedipine. These results indicate that K+ modulates elastin and collagen synthesis and their gene expression reciprocally, and these effects are mediated by Ca2+ influx. Thus K+ exerts profound effects on the composition of extracellular matrices in aorta.