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Resistance of Morpholino Phosphorodiamidate Oligomers to Enzymatic Degradation

1996; Mary Ann Liebert, Inc.; Volume: 6; Issue: 4 Linguagem: Inglês

10.1089/oli.1.1996.6.267

2168-6599

Robert M. Hudziak, Elisabeth Barofsky, Douglas F. Barofsky, Doreen L. Weller, Shuo Huang, Dwight D. Weller,

CRISPR and Genetic Engineering

Oligomers possessing the Morpholino phosphorodiamidate backbone were evaluated for resistance to a variety of enzymes and biologic fluids. A 25-mer was incubated with nucleases, proteases, esterases, and serum, and the reaction mixtures were directly analyzed by MALDI-TOF mass spectrometry. The 25-mer was completely resistant to 13 different hydrolases and serum and plasma. The excellent resistance of the Morpholino phosphorodiamidates to enzymatic attack indicates their suitability for in vivo use.