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Protective effect of Lepidium sativum against doxorubicin-induced nephrotoxicity in rats.

2010; Volume: 1; Issue: 3 Linguagem: Inglês

0975-8585

Nilesh Shinde, Amit Jagtap, Vaishali Undale, Sujit Kakade ., Sachin Kotwal, Ravindra Patil,

Plant tissue culture and regeneration

Nephrotoxicity is one of the important side effects of anthracycline antibiotics. The aim of this study was to investigate the effects of aqueous extract of Lepidium sativum L. against nephrotoxicity induced by doxorubicin (DXN).The rats were divided into control, Lepidium sativum (LS) alone, doxorubicin (15 mg/kg, i.p.) and doxorubicin plus LS (200 mg/kg, p.o.) and doxorubicin plus LS (400 mg/kg, p.o.) groups. At the end of the 72 hr, kidney tissues were removed for light microscopy and analysis. The levels of tissues malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) were determined. Serum creatinine and blood urea nitrogen were also measured. The serum urea and creatinine levels in the DXN alone treated group were significantly elevated (P<0.001) with respect to normal group of animals. The levels were reduced in the Lepidium sativum (200 and 400 mg/kg, p.o) treated groups. The renal antioxidant enzymes such as superoxide dismutase, catalase activities and level of reduced glutathione were declined; level of malondialdehyde was elevated in the DXN alone treated group. The activities of SOD, CAT and level of GSH were elevated and level of MDA declined significantly in the Lepidium sativum (200 and 400 mg/kg) plus DXN. Additionally, histopathological examination and scoring showed that Lepidium sativum markedly ameliorated DXN-induced renal tubular necrosis. Lepidium sativum can be considered a feasible candidate to protect against nephrotoxicity commonly encountered with doxorubicin treatment.