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An Improved Method for the Artificial Insemination of the Mouse (Mus musculus)1

1973; Oxford University Press; Volume: 9; Issue: 4 Linguagem: Inglês

10.1093/biolreprod/9.4.420

1529-7268

Peter A. Leckie, J. Gary Watson, Sterling Chaykin,

Reproductive Biology and Fertility

A technique has been devised for the artificial insemination of mice (C3HeB/FeJ) which does not require the participation of a vasectomized male. The use of an artificial penis and a vaginal tampon have been shown to be satisfactory substitutes for the vasectomized males. When both the artificial penis and the vaginal tampon were used 69–87% of the inseminated animals successfully implanted embryos. The implantation percentages when the artificial penis and the vaginal tampon were used separately were 31 and 50, respectively.