Study on the Effects of Various Doses of Tribulus Terrestris Extract on Epididymal Sperm Morphology and Count in Rat
2013; Volume: 10; Issue: 1 Linguagem: Inglês
ISSN
1999-8163
AutoresRamin Kaffashi Elahi, Sirousrafiy Asl, Farhad Shahian, Tabriz Branch,
Tópico(s)Phytochemical and Pharmacological Studies
Resumo2 Abstract: Tribulus Terrestris (TT) is a plant of tropical regions, traditionally used as aphrodisiac, analgesic and diuretic agent. Studies showed that TT extract produces significant aphrodisiac effects and increases testosterone levels. Nowadays TT is a major part of most aphrodisiac drugsand its cardiac effects in long time use are unclear. The purpose was assessment of effects of various doses of Tribulus Terrestris extract on epididymal sperm morphology and its quantity in rat. 21 male wistar rats aged 3-4 month-old weighting 250-350 g were selected by chance. Oral solution of Tribulus Terrestris was prepared and after approving by pharmaceutical center of Tabriz University and after the 2 weeks adaptation was administrated at the dose of 5mg/kg daily. Rats were divided into the 3 identical groups of 7 rats in each. Treatment 1 received normal dose of drug (5 mg/kg) and treatment 2 received the double dose of drug. At the end, rats were constrained and anesthetized by xylazine and ketamine intraperitoneally at the dose of 5 and 60 mg/kg respectively. One of the testis and epididymis were removed at once. The contents of epididymis were extracted by solution Ham's F-10 and transferred on the plates which were in the incubator. Then, plates were stained by Eosin and Nigrosin method and assayed microscopically. Results showed the effectiveness of Tribulus Terrestris extract on sexual dysfunction in experimental rats. On the basis of our results, we concluded that Tribulus Terrestris extract has the potential to be used as a safe therapeutic alternative to current modalities for the management of sexual dysfunction in males.
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