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Rationales for expression and altered expression of apoptotic protease activating factor-1 gene in gastric cancer

2007; Baishideng Publishing Group; Volume: 13; Issue: 38 Linguagem: Inglês

10.3748/wjg.v13.i38.5060

2219-2840

Heling Wang,

Protease and Inhibitor Mechanisms

AIM:To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS:Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study.Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR.Loss of heterozygosity (LOH) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples.Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR. RESULTS:The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%.The LOH frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively.Fifty percent LOH was found at two sites and 17% LOH at three sites.Apaf-1 mRNA expression decreased significantly in 13 cases (r s = 0.487, P = 0.003).The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples.Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA r s = 0.886, P = 10 -6 ). CONCLUSION:The expression of Apaf-1 gene is low in gastric cancer tissues.Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.