Produção Nacional
Revisado por pares
First Report of Grapevine Dieback Caused by Neoscytalidium hyalinum in Brazil
2015; American Phytopathological Society; Volume: 100; Issue: 1 Linguagem: Inglês
10.1094/pdis-03-15-0366-pdn
ISSN1943-7692
AutoresKamila C. Correia, M. A. Silva, Mariote Santos Brito Netto, Willie Anderson dos Santos Vieira, Marcos Paz Saraiva Câmara, Sami Jorge Michereff,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 100, No. 1First Report of Grapevine Dieback Caused by Neoscytalidium hyalinum in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Grapevine Dieback Caused by Neoscytalidium hyalinum in BrazilK. C. Correia, M. A. Silva, M. S. B. Netto, W. A. S. Vieira, M. P. S. Câmara, and S. J. MichereffK. C. CorreiaSearch for more papers by this author, M. A. SilvaSearch for more papers by this author, M. S. B. NettoSearch for more papers by this author, W. A. S. VieiraSearch for more papers by this author, M. P. S. CâmaraSearch for more papers by this author, and S. J. MichereffSearch for more papers by this authorAffiliationsAuthors and Affiliations K. C. Correia , Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Crato, 63133-610, Ceará, Brazil M. A. Silva M. S. B. Netto W. A. S. Vieira M. P. S. Câmara S. J. Michereff , Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil. Published Online:11 Nov 2015https://doi.org/10.1094/PDIS-03-15-0366-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In May 2013, grapevine (Vitis vinifera L.) green shoots and canes showing dieback symptoms were collected during a survey conducted in 14 vineyards of São Francisco Valley, northeastern Brazil. The dieback symptoms were prevalent in 40.9% of vineyards surveyed, mainly in mature plants. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g/liter streptomycin sulfate. Conidial characters of three isolates (CMM 314, CMM 371, and CMM 394) were observed after growth on 2% water agar bearing autoclaved pine needles for 3 weeks at 25°C under near-UV light. Conidia in arthric chains were found in cultures. Coelomycetous synasexual morph conidia were ellipsoid to ovoid, with a truncate base and an acutely rounded apex, initially aseptate, becoming brown and two-celled at maturity, 9.2 μm ± 2.1 × 3.9 μm ± 0.5 (n = 50), typical of Neoscytalidium species (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rRNA) region of three previously mentioned isolates were conducted to identify the species as described by Netto et al. (2014). Sequences of the isolates were 99% similar to those of N. hyalinum (C.K. Campb. & J.L. Mulder) A.J.L. Phillips, Groenewald & Crous (syn. N. dimidiatum) for EF1-α (GenBank Accession Nos. JX513617 and JX513618) and ITS (JX513637 and JX513638). A phylogenetic analysis using maximum parsimony and including published EF1-α and ITS data for Neoscytalidium species was carried out, showing that the isolated fungi belong to the N. hyalinum clade. Sequences of the isolates were deposited in GenBank (ITS, KF719953 to KF719955; EF1-α KF719956 to KF719958). Cultures were deposited in the Culture Collection of Phytopathogenic Fungi of the Universidade Federal Rural de Pernambuco, Recife, Brazil. Pathogenicity tests were performed with all three strains of N. hyalinum on detached green shoots of grape (cv. Isabel). Detached shoots (30 cm long) were wounded (∼4-mm length, 2-mm deep) between two nodes using a sterilized scalpel. Mycelial plugs from the margin of actively growing colonies (PDA) of each isolate were placed in the wounds. Noncolonized PDA plugs were used as control. The inoculated area was wrapped with Parafilm. Inoculated and control shoots (15 each/isolate) were maintained in a moist chamber for 24 h at 25°C and 12-h photoperiod. The experiment was repeated twice. After 10 days, all inoculated shoots showed necrotic lesions, with an average length of 6.3 cm (±1.4 cm). No symptoms were observed in the control shoots. The pathogen was reisolated from all inoculated shoots and identified by conidia morphology to fulfill Koch’s postulates. Neoscytalidium hyalinum has been identified as the causal agent of dieback on grapevine in Iran (Al-Saadoon et al. 2012) and the United States (Rolshausen et al. 2013). To our knowledge, this is the first report of N. hyalinum causing grapevine dieback in Brazil.References:Al-Saadoon, A. H., et al. 2012. Afr. J. Biotechnol. 11:16165. Crossref, Google ScholarNetto, M. S. B., et al. 2014. Fungal Divers. 67:127. Crossref, ISI, Google ScholarPhillips, A. J. L., et al. 2013. Stud. Mycol. 76:51. Crossref, ISI, Google ScholarRolshausen, P. E., et al. 2013. Plant Dis. 97:1511. Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 1 January 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Jan 2016Published: 11 Nov 2015First Look: 27 Aug 2015Accepted: 12 Aug 2015 Pages: 213-213 Information© 2016 The American Phytopathological SocietyCited byPhytotoxic Metabolites Produced by Fungi Involved in Grapevine Trunk Diseases: Progress, Challenges, and Opportunities5 December 2022 | Plants, Vol. 11, No. 23A review of research trends on Nattrassia mangiferae from 1966-2022: A bibliometric approach30 November 2022 | Scientific Research and Essays, Vol. 17, No. 4Neoscytalidium dimidiatumCABI Compendium, Vol. CABI CompendiumDiversity of Botryosphaeriaceae causing grapevine trunk diseases and their spatial distribution under different climatic conditions in Algeria20 September 2021 | European Journal of Plant Pathology, Vol. 161, No. 4Neoscytalidium dimidiatum causes leaf blight on Sansevieria trifasciata in Brazil23 April 2020 | Australasian Plant Disease Notes, Vol. 15, No. 1Fruit rot caused by Neoscytalidium hyalinum on melon in Iran16 March 2019 | Australasian Plant Disease Notes, Vol. 14, No. 1New disease caused by Neoscytalidium dimidiatum devastates tomatoes (Solanum lycopersicum) in TurkeyCrop Protection, Vol. 118Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches14 March 2018 | Fungal Diversity, Vol. 90, No. 1
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