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Enicostemma Littorale: A new therapeutic target for islet neogenesis

2010; Mary Ann Liebert, Inc.; Volume: 9; Issue: 1 Linguagem: Inglês

0973-8363

Sarita Gupta, Nidheesh Dadheech, Anubha Singh, Sanket Soni, Ramesh Bhonde,

Diabetes and associated disorders

Multipotent differentiation property of stem cells opens up new arena for the treatment of diabetic patients. Many chemical and biochemical compounds make stem cells to get differentiate into insulin producing cells. Present study highlighted islet neogenic property of one herbal plant Enicostemma littorale blume. An active herbal compound SGL-1 was isolated and purified from extract of Enicostemma littorale and used to differentiate two model stem cell lines PANC-1 and NIH3T3 which showed tremendous islet neogenic potential and significant islet yield compared to control serum free medium (SFM). Morphological, molecular and immunological characterization of newly generated islet like cellular aggregates (ICA's) proved them differentiated and positive for islet hormones. Functional characterization of ICA's confirmed significant glucose responsive insulin release. This preliminary data does offer exciting possibility of alternate source to increase islet mass which can be used for treatment of diabetic patients.