Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium
2015; Elsevier BV; Volume: 139; Linguagem: Inglês
10.1016/j.colsurfb.2015.11.051
ISSN1873-4367
AutoresSara Gonçalves, Inês Rodrigues, Jorge Padrão, João Pedro Silva, Vítor Sencadas, S. Lanceros‐Méndez, Henrique Girão, Miguel Gama, Fernando Dourado, L. R. Rodrigues,
Tópico(s)Corneal Surgery and Treatments
ResumoThis work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5–2.0 swelling/mm3), high mechanical strength (2048 MPa) and non-pyrogenicity (2.12 EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored.
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