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Association of the −1031T>C polymorphism and soluble TNF-α levels with Acute Coronary Syndrome

2015; Elsevier BV; Volume: 78; Linguagem: Inglês

10.1016/j.cyto.2015.11.014

1096-0023

Elena Sandoval‐Pinto, Jorge Ramón Padilla‐Gutiérrez, Emmanuel Valdés‐Alvarado, Ilian Janet García-González, Angélica Valdez-Haro, José Francisco Muñoz‐Valle, Héctor Enrique Flores-Salinas, Lorena Michele Brennan-Bourdon, Yeminia Valle,

NF-κB Signaling Pathways

Inflammation has gained a pivotal role in the pathophysiology of Acute Coronary Syndrome (ACS). TNF-α is a pro-inflammatory cytokine that could be a potential biomarker in ACS due to its multiple functions. The rs1799964 TNFA polymorphism (−1031 T > C) has been associated with a decrease in gene transcription and cytokine levels. To determine the association of rs1799964 TNFA polymorphism and TNF-α soluble levels in ACS. A total of 251 patients diagnosed with ACS and 164 individuals without cardiovascular diseases classified as the reference group (RG), were included. The rs1799964 polymorphism was genotyped by PCR-RFLP. Soluble protein levels were determined by ELISA. Statistical analyses were performed using chi square and U-Mann Whitney tests. The genotype and allele frequencies were different between ACS and RG (OR = 0.317, p = 0.01; OR = 0.688, p = 0.03 respectively). ACS patients had higher soluble TNF-α levels compared with the RG (31.08 vs 23.00 pg/mL, p < 0.001); according genotype significant differences were observed (T/T: 24.06 vs T/C: 34.95 pg/mL, p = 0.0001) in patients. In the RG, T/T carriers showed discrete lower levels than C/C genotype (22.14 vs 27.83 pg/mL, p = 0.04). The −1031 C allele of the TNFA polymorphism confers protection for the development of ACS. The T/C genotype carriers had higher TNF-α serum levels compared to the T/T genotype in ACS. In addition, the −1031 T > C TNFA polymorphism was associated with dyslipidemia in ACS in a Western Mexican population.