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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

High-performance liquid chromatographic analysis of bioactive quinones from Auxemma glazioviana

2004; ARKAT USA, Inc.; Volume: 2004; Issue: 6 Linguagem: Inglês

10.3998/ark.5550190.0005.610

ISSN

1551-7012

Autores

José Galberto Martins da Costa, Ângela M. C. Arriaga, Marcos Carlos de Mattos, Otília Deusdênia Loiola Pessoa, Ronaldo Ferreira do Nascimento, Telma L. G. Lemos,

Tópico(s)

Essential Oils and Antimicrobial Activity

Resumo

Chemical constituents obtained from ethanol extract of Auxemma glazioviana, a very common tree of Ceará State-Brazil, were analyzed and quantified by reverse-phase HPLC.To evaluate the quinone content of the extract, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed for the assessment of three bioactive quinones: glaziovianol 1, oncocalyxone A 2 and oncocalyxone C 3. The HPLC system used a Shimadzu octadecylsilyl silica (ODS) column with acetonitrile and water (1:1, v/v), as the mobile phase and detection at 280 nm.Quantitative analysis showed that 2 is the most abundant compound in both extracts EtOAc and CHCl 3 , with 35.9 and 39.9%, followed by 1 with 22.8 and 26.2%, respectively.

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