Produção Nacional
Revisado por pares
First Report on Detection of Plum pox virus in Imported Peach Fruits in Brazil
2015; American Phytopathological Society; Volume: 100; Issue: 4 Linguagem: Inglês
10.1094/pdis-09-15-1015-pdn
ISSN1943-7692
AutoresJorge Alberto Marques Rezende, Viviana M. Camelo-García, Elliot Watanabe Kitajima,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 100, No. 4First Report on Detection of Plum pox virus in Imported Peach Fruits in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report on Detection of Plum pox virus in Imported Peach Fruits in BrazilJ. A. M. Rezende, V. M. Camelo, and E. W. KitajimaJ. A. M. RezendeSearch for more papers by this author, V. M. CameloSearch for more papers by this author, and E. W. KitajimaSearch for more papers by this authorAffiliationsAuthors and Affiliations J. A. M. Rezende V. M. Camelo E. W. Kitajima , ESALQ/University of São Paulo, Department of Plant Pathology and Nematology, Piracicaba, São Paulo, Brazil. Published Online:17 Feb 2016https://doi.org/10.1094/PDIS-09-15-1015-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In April 2014, we found peach fruits displaying symptoms of ringspots in a fruit and vegetable shop in the city of Piracicaba, São Paulo State, Brazil. The fruits were imported from Chile, where the occurrence of Plum pox virus (PPV) was first reported in apricots, plums, and peaches by Rosales et al. (1997). Extract from symptomatic fruit, examined by transmission electron microscopy, revealed the presence of elongated and flexuous particles characteristic of potyvirus. Sap from three symptomatic fruits reacted in DAS-ELISA with polyclonal antiserum against PPV (BIOREBA AG, Switzerland). Total RNA was extracted from three symptomatic fruits according to Toth et al. (2002) and subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) with the pair of primers PV1 and WCIEN-sense (Maciel et al. 2011), which amplifies a fragment of approximately 700 nt containing part of the capsid protein gene and the 3′ untranslated region of potyviruses. The amplicons were directly sequenced in both directions at Macrogen (Seoul, South Korea). Comparison of the nucleotide (645 nt) and deduced amino acid (215 aa) sequences of the three amplicons (GenBank Accession Nos. KT595211, KT595212, and KT595213) showed 99% and 100% identity, respectively, with the corresponding sequences of a Chilean isolate of PPV (AF440741). Compared with corresponding sequences for other PPV isolates identities were 99% and 98 to 100%, respectively. This is the first finding in Brazil of this potyvirus, which is a quarantine pathogen. Imports of peach fruits infected with this potyvirus is a risk, albeit with a low probability of introduction of the virus into peach plants or any other Prunus sp. grown in the country. Gildow et al. (2004) showed experimentally that when Myzus persicae, Aphis spiraecola, A. fabae, and Brachycaudus persicae were given a 3-day probing access period on PPV-infected peach fruit and transferred to healthy peach seedlings, they transmitted PPV to 50, 35, 0, and 0% of seedlings, respectively. These results suggest that PPV-infected fruit is potentially a virus source for long-distance dispersal. This occurrence was communicated to the Brazilian Ministry of Agriculture for possible regulations on imports of Prunus sp. fruits infected with PPV.References:Gildow, F., et al. 2004. Phytopathology 94:868. https://doi.org/10.1094/PHYTO.2004.94.8.868 Link, ISI, Google ScholarMaciel, S. C., et al. 2011. Sci. Agric. (Piracicaba, Braz.) 68:687. https://doi.org/10.1590/S0103-90162011000600013 Crossref, ISI, Google ScholarRosales, M., et al. 1997. Acta Hortic. 472:401. Google ScholarToth, I. K., et al. 2002. Methods for the Detection and Quantification of Erwinia carotovora subsp. atroseptica (Pectobacterium carotovorum subsb. atrosepticum) on Potatoes: A Laboratory Manual. Scottish Crop Research Institute, Dundee, Scotland. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 4 April 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 23 Mar 2016Published: 17 Feb 2016First Look: 1 Dec 2015Accepted: 20 Nov 2015 Pages: 869-869 Information© 2016 The American Phytopathological SocietyCited byPlum pox virus (sharka)CABI Compendium, Vol. CABI CompendiumPrunus persica (Peach)6 June 2020Sharka, a vector-borne disease caused by Plum pox virus : vector species, transmission mechanism, epidemiology and mitigation strategies to reduce its natural spreadActa Horticulturae, No. 1163Identification of Plum pox virus causing sharka disease on peach ( Prunus persica L.) in Mexico22 March 2017 | Canadian Journal of Plant Pathology, Vol. 39, No. 1Western Flower Thrips Can Transmit Tomato spotted wilt virus From Virus-infected Tomato FruitsStephanie A. Szostek, Priscila Rodriguez, Jasmin Sanchez, Scott Adkins, and Rayapati A. Naidu16 March 2017 | Plant Health Progress, Vol. 18, No. 1
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