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Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection

2015; European Centre for Disease Prevention and Control; Volume: 20; Issue: 41 Linguagem: Inglês

10.2807/1560-7917.es.2015.20.41.30042

1560-7917

Sang‐Won Park, Ranawaka A. P. M. Perera, Pyoeng Gyun Choe, Eric H. Y. Lau, Seong Jin Choi, June Young Chun, Hong Sang Oh, Kyoung‐Ho Song, Ji Hwan Bang, Eu Suk Kim, Hong Bin Kim, Wan Beom Park, Nam Joong Kim, Leo L. M. Poon, Malik Peiris, Myoung‐don Oh,

COVID-19 Clinical Research Studies

Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.