First Report of Tomato leaf curl Gujarat virus , a Bipartite Begomovirus on Cotton Showing Leaf Curl Symptoms in Pakistan
2015; American Phytopathological Society; Volume: 99; Issue: 11 Linguagem: Inglês
10.1094/pdis-02-15-0195-pdn
ISSN1943-7692
AutoresSyed Shan‐e‐Ali Zaidi, Zafar Iqbal, Imran Amin, Shahid Mansoor,
Tópico(s)Mosquito-borne diseases and control
ResumoHomePlant DiseaseVol. 99, No. 11First Report of Tomato leaf curl Gujarat virus, a Bipartite Begomovirus on Cotton Showing Leaf Curl Symptoms in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato leaf curl Gujarat virus, a Bipartite Begomovirus on Cotton Showing Leaf Curl Symptoms in PakistanS. S. A. Zaidi, Z. Iqbal, I. Amin, and S. MansoorS. S. A. Zaidi, Z. Iqbal, I. Amin, and S. MansoorAffiliationsAuthors and Affiliations S. S. A. Zaidi , Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, and Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan Z. Iqbal I. Amin S. Mansoor , Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. Published Online:8 Sep 2015https://doi.org/10.1094/PDIS-02-15-0195-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Cotton leaf curl is one of the most important constraints on cotton (Gossypium hirsutum) in Pakistan and northwestern India. The disease is associated with several monopartite begomoviruses, which essentially require a specific DNA satellite called Cotton leaf curl Multan betasatellite. The disease complex is evolving rapidly by component capture and recombination, which supposedly helps the complex to overcome resistance (Sattar et al. 2013). During a routine survey for monitoring cotton leaf curl disease in October 2013, several leaf samples of infected cotton plants showing typical symptoms of disease such as leaf curling and vein thickening were collected from the Gojra region of Punjab, and were checked for the presence of begomoviruses and associated satellites. Total DNA extracted from infected plants was resolved on agarose gel, transferred to nylon membrane, and probed with Cotton leaf curl Burewala virus probe. The probe detected begomovirus DNA forms found in two out of six infected plants and confirmed the association of begomovirus with the disease. Universal primers for begomoviruses (Akhtar et al. 2009) were used in PCR for confirmation of begomoviruses, and PCR products of expected size (∼2.8 kb) were obtained. For the detection of begomoviruses, the amplified products were cloned in a TA cloning vector and were sequenced. The complete nucleotide sequences of two clones showed 99% sequence identity with Tomato leaf curl Gujarat virus (ToLCGuV), available in database under accession numbers LN794214 and LN794215, a bipartite begomovirus reported from India (Chakraborty et al. 2003). ToLCGuV was previously found on a weed species in Pakistan but effort to find cognate DNA B was not successful (Mubin et al. 2012). In order to confirm the bipartite nature of the virus, specific primers were designed on DNA B (forward primer 5′-GGTACCCGTAACGATCTTGAACTATGTCCC-3′ and reverse primer 5′-GGTACCCTATCTGGCTATAGGTCCGAACG-3′). The use of these primers in PCR resulted in amplification of a full length ∼2.7 kb product, confirming the bipartite nature of virus. PCR analysis of 40 plant samples was done, among which 14 showed amplification of expected size. The amplified product was cloned and fully sequenced. The sequence analysis confirmed the identity as DNA B of Tomato leaf curl New Delhi virus (ToLCNDV), available in database under accession number LN713269, which is 92% identical to the ToLCNDV DNA B isolated from Sonchus arvensis, a weed found in Pakistan (Mubin et al. 2010). To our knowledge, this is the first report of a bipartite begomovirus on cotton showing leaf curl disease symptoms; however, further work is needed to confirm Koch's postulate on cotton. The association of a bipartite virus may help the virus complex to overcome host disease resistance or expansion of the host range.References:Akhter, A., et al. 2009. Plant Dis. 93:962. https://doi.org/10.1094/PDIS-93-9-0962B Link, ISI, Google ScholarChakraborty, S., et al. 2003. Phytopathology 93:1485. https://doi.org/10.1094/PHYTO.2003.93.12.1485 Link, ISI, Google ScholarMubin, M., et al. 2010. Virus Genes 40:452. https://doi.org/10.1007/s11262-010-0470-y Crossref, ISI, Google ScholarMubin, M., et al. 2012. Virus Genes 44:112. https://doi.org/10.1007/s11262-011-0662-0 Crossref, ISI, Google ScholarSattar, M. N., et al. 2013. J. Gen. Virol. 94:695. https://doi.org/10.1099/vir.0.049627-0 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 11 November 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 4 Nov 2015Published: 8 Sep 2015First Look: 4 May 2015Accepted: 29 Apr 2015 Page: 1655 Information© 2015 The American Phytopathological SocietyCited byEmergence of Asian endemic begomoviruses as a pandemic threat28 September 2022 | Frontiers in Plant Science, Vol. 13Cotton leaf curl disease complex (leaf curl disease of cotton)CABI Compendium, Vol. CABI CompendiumTomato leaf curl Gujarat virusCABI Compendium, Vol. CABI CompendiumMolecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton ( Gossypium hirsutum )30 September 2019 | Plant Biotechnology Journal, Vol. 18, No. 3Cotton Diseases and Their Management6 March 2020Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites14 May 2019 | Plants, Vol. 8, No. 5Evolutionary Factors in the Geminivirus Emergence13 July 2019Gossypium spp. (Cotton)6 June 2020Bemisia tabaci-mediated facilitation in diversity of begomoviruses: Evidence from recent molecular studiesMicrobial Pathogenesis, Vol. 123Association of tomato leaf curl Gujarat virus and tomato leaf curl Bangladesh betasatellite on papaya showing typical leaf curl symptoms in North India8 May 2018 | 3 Biotech, Vol. 8, No. 5Identification of a new begomovirus infecting Duranta repens in Pakistan9 December 2017 | Archives of Virology, Vol. 163, No. 3Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton6 April 2017 | Scientific Reports, Vol. 7, No. 1An Insight into Cotton Leaf Curl Multan Betasatellite, the Most Important Component of Cotton Leaf Curl Disease Complex29 September 2017 | Viruses, Vol. 9, No. 10Tomato leaf curl New Delhi virus : a widespread bipartite begomovirus in the territory of monopartite begomoviruses17 October 2016 | Molecular Plant Pathology, Vol. 18, No. 7The Prediction of a New CLCuD Epidemic in the Old World19 April 2017 | Frontiers in Microbiology, Vol. 8Status and Diversity of Begomoviruses in Pakistan15 October 2017Engineering Molecular Immunity Against Plant VirusesFrequent Occurrence of Tomato Leaf Curl New Delhi Virus in Cotton Leaf Curl Disease Affected Cotton in Pakistan23 May 2016 | PLOS ONE, Vol. 11, No. 5
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