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First Report of Micrococcus luteus Causing Leafspot on Mangifera indica in Rajkot, India

2015; American Phytopathological Society; Volume: 99; Issue: 11 Linguagem: Inglês

10.1094/pdis-12-14-1359-pdn

1943-7692

Purvi M. Rakhashiya, Pooja P. Patel, Vrinda S. Thaker,

Phytoplasmas and Hemiptera pathogens

HomePlant DiseaseVol. 99, No. 11First Report of Micrococcus luteus Causing Leafspot on Mangifera indica in Rajkot, India PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Micrococcus luteus Causing Leafspot on Mangifera indica in Rajkot, IndiaP. M. Rakhashiya, P. P. Patel, and V. S. ThakerP. M. RakhashiyaSearch for more papers by this author, P. P. PatelSearch for more papers by this author, and V. S. ThakerSearch for more papers by this authorAffiliationsAuthors and Affiliations P. M. Rakhashiya P. P. Patel V. S. Thaker , Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering (CPBGE), Department of Biosciences, Saurashtra University, Rajkot 360005, Gujarat, INDIA. Published Online:21 Sep 2015https://doi.org/10.1094/PDIS-12-14-1359-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Mango (Mangifera indica L.) is commercially grown in more than 80 countries of the world, among which India ranks first (Gagnevin and Pruvost 2001). A black leafspot disease of unknown etiology causes considerable leaf fall, reduces fruit set, and ultimately leads to fruit loss in the mango production regions in India. Mangifera indica leaves with black leafspot symptoms were collected in August 2013 from near Rajkot City (22.30°N, 70.78°E), Gujarat, India. Leafspots were dark brown at the periphery, black in the center, and irregular in size and shape. Sampled leaves were washed, surface sterilized, and incubated on nutrient agar (NA: 10 g/liter beef extract, 10 g/liter peptone, 5 g/liter sodium chloride, 20 g/liter agar, pH 7.3; HiMedia, Mumbai, India) at 27°C for 24 to 48 h. Bacterial colonies were isolated from the infected tissue. Colonies were yellow, opaque, and convex with a smooth texture. Bacterial cells were coccoid and clustered in groups of four. The isolated bacterial pathogen was identified as Micrococcus luteus based on morphological characteristics (Bergey’s Manual of Systematic Bacteriology) and sequencing of the 16s rDNA (Srinivasa et al. 2012). A bacterial suspension (106 CFU/ml) was injected into the midrib of about 25 leaves from five plants and incubated for 1 month at 37°C in glasshouse (Cazorla et al. 1998), along with five noninoculated control plants. Typical leafspot symptoms developed on inoculated plants, but not on controls. Micrococcus luteus were consistently isolated from all infected leaves, proving pathogenicity and confirming Koch’s postulates. The conserved region of 16S rRNA genes from both isolated and reisolated bacteria was amplified by using the universal primer set 8F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1512R (5′-ACGGCTACCTTGTTACGACTT-3′). Sequences were submitted to GenBank under Accession Nos. KM454981 and KM37667. Although several bacterial pathogens are known to cause various diseases in M. indica (Buruchara et al. 1990), this is, to the best of our knowledge, the first report of M. luteus SUBG006 causing leafspot on M. indica in Rajkot, Gujarat, India.References:Buruchara, R. A., et al. 1990. Page 577 in: 7th Int. Conf. Plant. Path. Bact. Akadémiai Kiadó, Budapest, Hungary. Google ScholarCazorla, F. M., et al. 1998. Phytopathology 88:614. https://doi.org/10.1094/PHYTO.1998.88.7.614 Link, ISI, Google ScholarGagnevin, L., and Pruvost, O. 2001. Plant Dis. 85:928. https://doi.org/10.1094/PDIS.2001.85.9.928 Link, ISI, Google ScholarSrinivasa, C., et al. 2012. Acta Biochim. Biophys. Sin. (Shanghai) 44:217. https://doi.org/10.1093/abbs/gmr129 Crossref, ISI, Google ScholarAuthors are thankful to the Department of Biosciences, Saurashtra University, and Financial Assistance Programme (FAP-2010), GSBTM for providing required facilities to conduct the research. Author P. M. Rakhashiya is thankful to University Grant Commission for providing grant in the form of BSR fellowship (RFSMS).DetailsFiguresLiterature CitedRelated Vol. 99, No. 11 November 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 4 Nov 2015Published: 21 Sep 2015Accepted: 30 Apr 2015 Page: 1640 Information© 2015 The American Phytopathological SocietyCited byMangrove endophytes and their natural metabolites: role in promoting plant healthMicrococcus luteusCABI Compendium, Vol. CABI CompendiumFirst Report of Shot Hole Disease on Cherry Laurel (Prunus laurocerasus) Caused by Micrococcus aloeverae in IrelandL. Smith, H. A. Y. Gibriel, C. Brennan, M. del Pino de Elias, A. Twamley, F. Doohan, H. Grogan, and A. Feechan13 August 2020 | Plant Disease, Vol. 104, No. 10