Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization
2015; American Chemical Society; Volume: 137; Issue: 49 Linguagem: Inglês
10.1021/jacs.5b11014
ISSN1943-2984
AutoresGiang K. T. Nguyen, Antony Kam, Shining Loo, Anna Jansson, Lucy Xin Pan, James P. Tam,
Tópico(s)Microbial Natural Products and Biosynthesis
ResumoMacrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields.
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