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Sequential Notch activation regulates ventricular chamber development

2015; Nature Portfolio; Volume: 18; Issue: 1 Linguagem: Inglês

10.1038/ncb3280

1476-4679

Gaetano D’Amato, Guillermo Luxán, Gonzalo del Monte‐Nieto, Beatriz Martínez‐Poveda, Carlos Torroja, Wencke Walter, Matthew S. Bochter, Rui Benedito, Susan E. Cole, Fernando Martínez, Anna‐Katerina Hadjantonakis, Akiyoshi Uemura, Luis Jesús Jiménez‐Borreguero, José Luís de la Pompa,

Cardiac Fibrosis and Remodeling

Ventricular chambers are essential for the rhythmic contraction and relaxation occurring in every heartbeat throughout life. Congenital abnormalities in ventricular chamber formation cause severe human heart defects. How the early trabecular meshwork of myocardial fibres forms and subsequently develops into mature chambers is poorly understood. We show that Notch signalling first connects chamber endocardium and myocardium to sustain trabeculation, and later coordinates ventricular patterning and compaction with coronary vessel development to generate the mature chamber, through a temporal sequence of ligand signalling determined by the glycosyltransferase manic fringe (MFng). Early endocardial expression of MFng promotes Dll4–Notch1 signalling, which induces trabeculation in the developing ventricle. Ventricular maturation and compaction require MFng and Dll4 downregulation in the endocardium, which allows myocardial Jag1 and Jag2 signalling to Notch1 in this tissue. Perturbation of this signalling equilibrium severely disrupts heart chamber formation. Our results open a new research avenue into the pathogenesis of cardiomyopathies. De la Pompa and colleagues show that Dll4-mediated Notch signalling regulates trabeculation in the developing mouse endocardium, whereas Jag1/2-mediated Notch signalling in the myocardium drives compaction and ventricular maturation.