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First Report of Potato mop-top virus in Chile

2015; American Phytopathological Society; Volume: 100; Issue: 6 Linguagem: Inglês

10.1094/pdis-07-15-0816-pdn

1943-7692

E. Peña, M. Carmen Domínguez Gutiérrez, Alejandro Montecinos, M. Muñoz, E. Vargas, Ivette Acuña, Rodrigo A. Gutiérrez, I. M. Rosales,

Plant and Fungal Interactions Research

HomePlant DiseaseVol. 100, No. 6First Report of Potato mop-top virus in Chile PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Potato mop-top virus in ChileE. Peña, M. Gutiérrez, A. Montecinos, M. Muñoz, E. Vargas, I. Acuña, R. A. Gutiérrez, and I. M. RosalesE. PeñaSearch for more papers by this author, M. GutiérrezSearch for more papers by this author, A. MontecinosSearch for more papers by this author, M. MuñozSearch for more papers by this author, E. VargasSearch for more papers by this author, I. AcuñaSearch for more papers by this author, R. A. GutiérrezSearch for more papers by this author, and I. M. RosalesSearch for more papers by this authorAffiliationsAuthors and Affiliations E. Peña , Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile M. Gutiérrez , Servicio Agrícola y Ganadero, Osorno, Chile A. Montecinos , Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile M. Muñoz E. Vargas , Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile I. Acuña , Centro Regional Remehue, Instituto de Investigaciones Agropecuarias, Osorno, Chile R. A. Gutiérrez , Millennium Nucleus Center for Plant Functional Genomics, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile I. M. Rosales , Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile. Published Online:8 Mar 2016https://doi.org/10.1094/PDIS-07-15-0816-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Potato mop-top virus (PMTV) is an important pathogen of potato (Solanum tuberosum L.), occurring in Northern Europe, North and South America, and Asia, and significantly reducing potato production (Santala et al. 2010). PMTV is transmitted by spores of Spongospora subterranea f. sp. subterranea, the causal agent of powdery scab of potato (Jones and Harrison 1969). During the summer season of 2012, native potato (S. tuberosum L. ssp. tuberosum) leaves showing virus-like symptoms were collected on several points within the Chiloé Archipelago (Chiloé Province, southern Chile), one of the centers of origin and diversity of cultivated potatoes (Montaldo 1984). The presence and identity of viruses in the samples were determined through next-generation high-throughput sequencing (Solexa-Illumina) followed by assembly of viral small RNAs (vsRNAs) to reference plant virus genomes. The plant material used for the construction of the sRNA library corresponded to pools of symptomatic leaves sampled in the Archipelago. This analysis revealed that PMTV was infecting native potatoes in Chiloé, since the assembled contigs covered 77.3, 76.8, and 68.0% of PMTV RNA1 (reference sequence, GenBank Accession No. NC_003723.1), RNA2 (reference sequence, NC_003725.1) and RNA3 (reference sequence, NC_003724.1), respectively. Subsequently, three farms growing native potatoes (local varieties Michuñe negra, Michuñe roja, and Clavela lisa) with symptoms associated with PMTV infections were identified in Puqueldón (Lemuy Island, Chiloé Province) during the summers of 2013 and 2014. Disease symptoms observed included stunting of stems, shortening of internodes, and mosaic patterns (V-shaped) on leaves, but necrotic symptoms on the surface or the flesh of tubers were not observed. To detect PMTV, total nucleic acid was extracted from three symptomatic plants each year and tested by reverse transcription (RT)-PCR using two sets of primers. In all samples analyzed, primer set C819/H360, targeting the coat protein (CP) gene in RNA3 yielded an 460-bp amplicon (Xu et al. 2004). Primer set pmtF4/pmtR4 amplified a 417-bp fragment from the second gene of the triple gene block (TGB2) in RNA2 (Xu et al. 2004). PCR products were directly sequenced (Accession Nos. KT267163 and KT267164). BLAST analysis showed that the amplicons were 99 to 100% identical to most of the CP or TGB2 sequences from several PMTV isolates from Europe and the United States. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA, Bioreba AG) on the same symptomatic potato leaf samples confirmed the presence of the virus in all cases. To our knowledge, this is the first report of PMTV in Chile. However, the powdery scab pathogen, S. subterranea f. sp. subterranea, is widespread in the potato-production areas near Chiloé Province. These results provide important and cautionary information to be considered by programs managing seed tuber production and plant quarantine for potato pathogens in Chile.References:Jones, R. A. C., and Harrison, B. D. 1969. Ann. Appl. Biol. 63:1. https://doi.org/10.1111/j.1744-7348.1969.tb05461.x Crossref, ISI, Google ScholarMontaldo, A. 1984. Cultivo y mejoramiento de la papa. Instituto Interamericano de Cooperación para la Agricultura, San José, Costa Rica. Google ScholarSantala, J., et al. 2010. Ann. Appl. Biol. 157:163. https://doi.org/10.1111/j.1744-7348.2010.00423.x Crossref, ISI, Google ScholarXu, H., et al. 2004. Plant Dis. 88:363. https://doi.org/10.1094/PDIS.2004.88.4.363 Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 6 June 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 12 May 2016Published: 8 Mar 2016First Look: 8 Dec 2015Accepted: 30 Nov 2015 Pages: 1250-1250 Information© 2016 The American Phytopathological SocietyCited bySpongospora subterranea f.sp. subterranea (powdery scab)CABI Compendium, Vol. CABI CompendiumPotato mop-top virus (potato mop-top)CABI Compendium, Vol. CABI CompendiumSolanum tuberosum (Potato)6 June 2020