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First Report of Fusarium decemcellulare Causing Inflorescence Wilt and Vascular and Flower Necrosis of Rambutan ( Nephelium lappaceum ), Longan ( Dimocarpus longan ), and Mango ( Mangifera indica )

2015; American Phytopathological Society; Volume: 99; Issue: 8 Linguagem: Inglês

10.1094/pdis-09-14-0923-pdn

1943-7692

L. M. Serrato-Diaz, M. Perez-Cuevas, Lydia I. Rivera-Vargas, Ricardo Goenaga, R. D. French-Monar,

Yeasts and Rust Fungi Studies

HomePlant DiseaseVol. 99, No. 8First Report of Fusarium decemcellulare Causing Inflorescence Wilt and Vascular and Flower Necrosis of Rambutan (Nephelium lappaceum), Longan (Dimocarpus longan), and Mango (Mangifera indica) PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium decemcellulare Causing Inflorescence Wilt and Vascular and Flower Necrosis of Rambutan (Nephelium lappaceum), Longan (Dimocarpus longan), and Mango (Mangifera indica)L. M. Serrato-Diaz, M. Perez-Cuevas, L. I. Rivera-Vargas, R. Goenaga, and R. D. French-MonarL. M. Serrato-DiazSearch for more papers by this author, M. Perez-CuevasSearch for more papers by this author, L. I. Rivera-VargasSearch for more papers by this author, R. GoenagaSearch for more papers by this author, and R. D. French-MonarSearch for more papers by this authorAffiliationsAuthors and Affiliations L. M. Serrato-Diaz , Department of Plant Pathology and Microbiology, Texas A&M AgriLife Extension Service, Amarillo, TX M. Perez-Cuevas , Department of Agronomy, University of ISA, Dominican Republic L. I. Rivera-Vargas , Department of Crops and Agro-Environmental Sciences, University of Puerto Rico-Mayaguez Campus R. Goenaga , USDA-ARS, Tropical Agriculture Research Station, Mayaguez, Puerto Rico R. D. French-Monar , Department of Plant Pathology and Microbiology, Texas A&M AgriLife Extension Service, Amarillo, TX. Published Online:26 Jun 2015https://doi.org/10.1094/PDIS-09-14-0923-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Longan, mango, and rambutan are very important fruit crops in Puerto Rico. During a disease survey in Puerto Rico conducted from 2008 to 2010, 50% of the inflorescences were affected with inflorescence wilt and flower and vascular necrosis in 70% of the fields of rambutan and longan at the USDA-ARS Research Farm in Isabela, three commercial orchards, and the Mango Germplasm Collection of the University of Puerto Rico’s Experiment Station in Juana Diaz. Diseased inflorescence from longan, mango, and rambutan were disinfested with 70% ethanol, rinsed with sterile water (sw), and transferred to acidified potato dextrose agar (APDA). Four isolates of Fusarium decemcellulare Brick, Jahrb. Vereinig. Angew (Fd) were identified morphologically using taxonomic keys (1,4). On APDA, colonies of Fd were dark pink to reddish, producing yellow sporodochia and droplet exudates on culture media. Macroconidia (n = 50) were hyaline, septate, fusiform, curved and extremely long, ranged from 60 to 76 µm long by 5 to 7 µm wide, with a rounded or blunt apical cell. Microconidia (n = 50) were hyaline, oval, 0 to 1 septate, produced in long chains, and ranged from 7.5 to 12.9 µm long by 2.0 to 4.7 µm wide. Chlamydospores were absent. Globose perithecia were 120 to 200 µm in diameter with clavate, unitunicate asci measuring 60 to 70 µm long and 11 to 12 µm wide. Asci contained four to six hyaline, reniform, 3-septate ascospores, and were 15 to 20 µm long and 5 to 7 µm wide. For molecular identification, the ITS region of rDNA using ITS5-ITS4 primers, and a fragment of elongation factor 1-alpha (EF1-α) gene using EF1728F-EF1986R primers were amplified by PCR, sequenced, and compared using BLASTN with Fusarium spp. submitted to the NCBI GenBank. Accession numbers of gene sequences were GU797410 to 11 and KF971882 to 83 for ITS region; and KF971884 to 87 for EF1-α. All sequences were 98 to 99% identical to other sequences of Fd submitted to GenBank, accessions HM054153, HQ897815, HM054059, EU849670 to 71, and AB674295. For each fungal isolate, pathogenicity tests were conducted on six healthy nondetached inflorescences of rambutan, longan, and mango using two trees of each fruit. Both needle-wounded and unwounded inflorescences were inoculated with conidial suspension (7.6 × 105 conidial/ml) from 15-day-old pure cultures of Fd grown in APDA. Inoculated inflorescences were kept in plastic bags for 8 days under field conditions. Untreated controls were inoculated with sw only. The test was repeated twice. Eight days after inoculation, isolates of Fd caused flower necrosis and inflorescence wilt on rambutan and longan, respectively. On mango, isolates of Fd caused vascular necrosis and death of flowers along the rachis. No symptoms were observed in untreated controls. F. decemcellulare was only reisolated from diseased inflorescences, fulfilling Koch’s postulates. F. decemcellulare has been associated with branch cankers, galls, and dieback of tropical trees such as avocado, cocoa, and mango (1–3). To our knowledge, this is the first time that Fd has been reported causing inflorescence wilt, vascular necrosis, and flower necrosis in longan, rambutan, and mango.References:(1) Leslie, J. F., and Summerell, B. A. 2006. Page 388 in: The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA. Crossref, Google Scholar(2) Pérez-Vicente, L., et al. 2012. Fitosanidad 16:19. Google Scholar(3) Ploetz, R. C. 2003. Diseases of mango. Page 327 in: Diseases of Tropical Fruit Crops. CABI Publishing, Wallingford, UK. Crossref, Google Scholar(4) Rossman, A. Y., et al. 1999. Stud. Mycol. 42:1. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 8 August 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 6 Aug 2015Published: 26 Jun 2015First Look: 24 Feb 2015Accepted: 10 Feb 2015 Page: 1187 Information© 2015 The American Phytopathological SocietyCited byFungal and Oomycete Diseases of Minor Tropical Fruit Crops11 April 2022 | Horticulturae, Vol. 8, No. 4Albonectria rigidiuscula (green point gall)CABI Compendium, Vol. CABI CompendiumBotryosphaeriaceae Fungi as Causal Agents of Dieback and Corky Bark in Rambutan and LonganL. M. Serrato-Diaz, A. Aviles-Noriega, A. Soto-Bauzó, L. I. Rivera-Vargas, R. Goenaga, and P. 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