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Candidatus Phytoplasma aurantifolia (16SrII Group) Associated with Witches’ Broom Disease of Bamboo ( Dendrocalamus strictus ) in India

2015; American Phytopathological Society; Volume: 100; Issue: 1 Linguagem: Inglês

10.1094/pdis-05-15-0534-pdn

1943-7692

Amit Yadav, Vipool Thorat, Yogesh S. Shouche,

Cocoa and Sweet Potato Agronomy

HomePlant DiseaseVol. 100, No. 1Candidatus Phytoplasma aurantifolia (16SrII Group) Associated with Witches' Broom Disease of Bamboo (Dendrocalamus strictus) in India PreviousNext DISEASE NOTES OPENOpen Access licenseCandidatus Phytoplasma aurantifolia (16SrII Group) Associated with Witches' Broom Disease of Bamboo (Dendrocalamus strictus) in IndiaA. Yadav, V. Thorat, and Y. ShoucheA. Yadav, V. Thorat, and Y. ShoucheAffiliationsAuthors and Affiliations A. Yadav V. Thorat Y. Shouche , Microbial Culture Collection, National Centre for Cell Science, Pashan, Pune 411021, India. Published Online:3 Nov 2015https://doi.org/10.1094/PDIS-05-15-0534-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Solid or Calcutta bamboo (Dendrocalamus strictus Nees., Poaceae) is found in the tropical regions of Indian subcontinent, Southeast Asia, and China. D. strictus is semelparous and has a flowering cycle of 20 to 65 years. It is one of the toughest bamboos in India and extensively used in making pulp and rayon for paper mills and construction of light-weight houses as well as furniture, making it a commercially significant plant species. Its young shoots are used as food, its leaves as fodder, and it is also used in traditional medicine (Nadgauda et al. 1993). Leaf samples from symptomatic bamboo plants were collected from five geographically separated plantations from Maharashtra State during September 2014 to January 2015. Observed symptoms were typical witches' broom phenotype with severe proliferative branching at nodal regions and reduction in leaf size, as described earlier by Suryanarayana et al. (2009), and which were suggestive of a phytoplasma infection. To confirm the presence of a phytoplasma, 1 g of leaf tissue from each of the symptomatic and asymptomatic plants were used for total DNA extraction using the CTAB method. The phytoplasma 16S rRNA gene was detected in all 16 collected symptomatic D. strictus samples using nested PCR with phytoplasma universal primers P1 and P7 followed by primers R16F2n/R16R2 (Smart et al. 1996) on agarose gel. No amplification was observed in template DNA isolated from asymptomatic plants. PCR fragments generated using R16F2n/1492R primers were sequenced directly using six different primers, namely R16F2n, R16R2, 343R, 704F, 907R, and 1492R. The obtained 16S rRNA gene sequence (GenBank Accession No. LN811707, 1337 bp) showed 99.4% homology with a strain WBDL belonging to Peanut Witches' broom group phytoplasma (Candidatus Phytoplasma aurantifolia, U15442) found in Catharanthus roseus (Asteraceae) when compared using the EzTaxon 16S rRNA database. The virtual RFLP pattern (Zhao et al. 2009) derived from the 16S rRNA sequence (LN811707) was found most similar to the reference pattern of the 16Sr group II, subgroup C (AJ293216), with a pattern similarity coefficient of 0.99. To our knowledge, this is the only report of Ca. Phytoplasma aurantifolia associated with witches' broom disease of D. strictus from India in addition to Ca. Phytoplasma asteris (AB242433) associated with Phyllostachys nigra (black bamboo) reported from Korea (Jung et al., 2006). While Suryanayana et al. (2009) reported symptoms of witches' broom disease on D. strictus from Karnataka, India; it was not clear whether observed symptoms were related to phytoplasma infection. Additionally, there was no evidence to establish the species of the disease-causing organism. Dendrocalamus strictus being an economically important plant, its susceptibility to phytoplasma diseases, especially involving vegetative propagation, can cause significant negative effect on yield and therefore socio-economic status of the population engaged in its cultivation. Diagnostic accreditation of bamboo species for phytoplasma diseases and management of phytoplasma insect vectors will help reduce its exposure to witches' broom disease.References:Jung, H., et al. 2006. J. Gen. Plant Pathol. 72:261. https://doi.org/10.1007/s10327-006-0275-y Crossref, Google ScholarNadgauda, R., et al. 1993. Tree Physiol. 13:401. https://doi.org/10.1093/treephys/13.4.401 Crossref, ISI, Google ScholarSmart, C., et al. 1996. Appl. Environ. Microbiol. 62:2988. Crossref, ISI, Google ScholarSuryanarayana V., et al. Ind. Phytoplathol., 62: 245. Google ScholarZhao, Y., et al. 2009. Int. J. Syst. Evol. Microbiol. 59:2582. https://doi.org/10.1099/ijs.0.010249-0 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 1 January 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Jan 2016Published: 3 Nov 2015First Look: 27 Jul 2015Accepted: 20 Jul 2015 Pages: 209-209 Information© 2016 The American Phytopathological SocietyCited byBambusicolous fungi, diseases, and insect pests of bambooGenetic diversity of phytoplasmas associated with several bamboo species in India6 April 2022 | Forest Pathology, Vol. 52, No. 3Draft genome sequence of 'Candidatus Phytoplasma australasia', strain SS02 associated with sesame phyllody disease7 April 2022 | 3 Biotech, Vol. 12, No. 5Phytoplasma aurantifolia (lime witches' broom phytoplasma)CABI Compendium, Vol. CABI CompendiumOur understanding about phytoplasma research scenario in India23 January 2021 | Indian Phytopathology, Vol. 74, No. 2Pest categorisation of the non‐EU phytoplasmas of tuber‐forming Solanum spp.EFSA Journal, Vol. 18, No. 12Genetic diversity of phytoplasma strains infecting chrysanthemum varieties in India and their possible natural reservoirs28 August 2020 | 3 Biotech, Vol. 10, No. 9Pest categorisation of the non‐EU phytoplasmas of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L.EFSA Journal, Vol. 18, No. 1The Distribution of Phytoplasmas in South and East Asia: An Emerging Threat to Grapevine Cultivation11 September 2019 | Frontiers in Plant Science, Vol. 10Witches' Broom disease of Bamboo - New report on economically important Bamboos in South India10 March 2019 | Journal of Non Timber Forest Products, Vol. 26, No. 1Pest categorisation of Witches' broom disease of lime (Citrus aurantifolia) phytoplasmaEFSA Journal, Vol. 15, No. 10New subgroup 16SrXI-F phytoplasma strain associated with sugarcane grassy shoot (SCGS) disease in IndiaInternational Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 2Association of 16SrII-C and 16SrII-D subgroup phytoplasma strains with witches' broom disease of Parthenium hysterophorus and insect vector Orosius albicinctus in India18 October 2015 | Australasian Plant Disease Notes, Vol. 10, No. 1