Chapter 3 Theory of chromatography
1983; Elsevier BV; Linguagem: Inglês
10.1016/s0301-4770(08)60864-7
ISSN0301-4770
AutoresCsaba Horváth, Wayne R. Melander,
Tópico(s)Protein purification and stability
ResumoThe theory of chromatography draws on most areas of physical chemistry and has many roots in chemical engineering science. Two of these areas are of particular interest: the equilibrium distribution of a substance between two phases that is responsible for retention and selectivity, and the axial dispersion in the column that controls column efficiency. The chapter presents both phenomena in a concise yet comprehensive manner and describes the various terms encountered in chromatographic theory. It focuses mainly on linear elution chromatography, which has been the most popular and successful chromatographic mode. However, displacement chromatography, which is reappearing after a score of years in oblivion, is also described. In order to simplify this introduction to the theory of chromatography, the treatment is focused on gas chromatography (GC) and high-performance liquid chromatography (HPLC)—the most advanced techniques. These two techniques are applicable to all of the other techniques because the essentials of the chromatographic process proper are same in all instances.
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