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FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO

2014; V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs; Volume: 15; Issue: 3 Linguagem: Inglês

10.15825/1995-1191-2013-3-66-72

2412-6160

В. А. Сургученко, А. С. Пономарева, Л. А. Кирсанова, G. N. Bubentsova, N. N. Skaletskij, V. I. Sevastianov,

Periodontal Regeneration and Treatments

Aim . The goal of this study was to demonstrate the possibility of in vitro formation of tissue-engineered const- ruct of cartilage on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose-derived mesenchymal stromal cells. Materials and methods. As a biodegradable matrix, the hetero- geneous composition of the implantable gel Sphero ® GEL (Biomir Service, Krasnoznamensk, Moscow Region) was selected. To generate cell-engineered construct of cartilaginous tissue human adipose-derived mesenchy- mal stromal cells were mixed with biopolymer matrix Sphero ® GEL and cultured in chondrogenesis medium. Results. It was revealed that human adipose-derived mesenchymal stromal cells with biopolymer matrix under chondrogenic conditions generate three-dimensional structures and produce cartilaginous extracellu- lar matrix components: collagen type II and glycosaminoglycans. Conclusion. It was discovered that 3-D structures formed in the process of culture of cell-engineered constructs, began to synthesize the components of the cartilage extracellular matrix. This indicates the initial stage of in vitro formation of tissue-engineered cartilage construct.