Produção Nacional
Revisado por pares
At-Home Bleaching: Color Alteration, Hydrogen Peroxide Diffusion and Cytotoxicity
2015; Fundação Odontológica de Ribeirão Preto; Volume: 26; Issue: 4 Linguagem: Inglês
10.1590/0103-6440201300380
ISSN1806-4760
AutoresLetícia Cunha Amaral Gonzaga de Almeida, Diana Gabriela Soares, Fernanda Almeida de Azevedo, Marjorie de Oliveira Gallinari, Carlos Alberto de Souza Costa, Paulo Henrique dos Santos, André Luíz Fraga Briso,
Tópico(s)Dental Anxiety and Anesthesia Techniques
Resumo<p>This study evaluated the color change, cytotoxicity and hydrogen peroxide (HP) diffusion caused by different home bleaching protocols: 10% carbamide peroxide (CP) for 3 or 1.5 h, 6% hydrogen peroxide for 1.5 h or 45 min. To quantify the peroxide penetration, disks of bovine teeth were placed in artificial pulp chambers (APCs) containing acetate buffer, which was collected for evaluation in a spectrophotometer. For analysis of cytotoxicity, specimens were adapted in APCs containing culture medium, which subsequently was applied on MDPC-23 odontoblast-like cells for 1 h. Cellular metabolism was evaluated by methyl tetrazolium (MTT) assay and the color change of the specimens was analyzed using the CIE L * a * b * system. The data were submitted to ANOVA and Fisher test (α=5%). The treatment with 10% CP for 3 h was the most effective, and 6% HP for 45 min produced the lowest color change. The groups 10% CP for 1.5 h and 6% HP for 45 min had the lowest trans-enamel dentinal HP penetration, and the 6% HP for 1.5 h had the highest. None of the protocols affected cellular metabolism and morphology. In conclusion, reduced peroxide exposure time reduced the bleaching result; higher HP diffusion did not mean higher effectiveness.</p>
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