[53] Adenosine 3′-phosphate 5′-phosphosulfate
1981; Academic Press; Linguagem: Inglês
10.1016/s0076-6879(81)77055-1
ISSN1557-7988
Autores Tópico(s)Biochemical and Molecular Research
ResumoResearch in the area of sulfate ester biosynthesis has been somewhat hindered by the unavailability of satisfactory methods for the large-scale preparation of adenosine 3′-phosphate 5′-phosphosulfate (PAPS). Although several methods have been described using either chemical or biological approaches, the techniques are difficult to implement and often provide product in low yield. The synthesis described in this chapter provides PAPS at high levels of purity and in quantities greater than 400 mg. The initial step is the preparation of adenosine 2′,3′-cyclic phosphate 5′-phosphate from a mixture of adenosine 2′,5′- and 3′,5′-bisphosphates by treatment with dicyclohexylcarbodiimide. The product is then sulfated with triethylamine-N-sulfonic acid. The treatment of the sulfate ester, thus, formed with immobilized ribonuclease T2 specifically cleaves the cyclic phosphate ester to the 3′-phosphate. PAPS generated in this manner is purified by chromatography on DEAE-cellulose.
Referência(s)