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18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model

2015; Impact Journals LLC; Volume: 7; Issue: 1 Linguagem: Inglês

10.18632/oncotarget.6645

1949-2553

Daisuke Hayasaka, Kodai Nishi, Takeshi Fuchigami, Kazuya Shiogama, Takanori Onouchi, Satoshi Shimada, Yutaka Tsutsumi, Kouichi Morita,

Vector-Borne Animal Diseases

// Daisuke Hayasaka 1,2 , Kodai Nishi 3 , Takeshi Fuchigami 4 , Kazuya Shiogama 5 , Takanori Onouchi 5 , Satoshi Shimada 1,2 , Yutaka Tsutsumi 5 and Kouichi Morita 1,2 1 Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan 2 Leading Graduate School Program, Nagasaki University, Sakamoto, Nagasaki, Japan 3 Department of Radioisotope Medicine, Atomic Bomb Diseases Institute, Nagasaki University, Sakamoto, Nagasaki, Japan 4 Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki, Japan 5 Department of Pathology, Fujita Health University School of Medicine, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, Japan Correspondence to: Daisuke Hayasaka, email: // Keywords : SFTSV, 18F-FDG PET imaging, mouse model, intestinal disorder, antiserum therapy, Immunology and Microbiology Section, Immune response, Immunity Received : August 31, 2015 Accepted : December 05, 2015 Published : December 17, 2015 Abstract Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease that causes fever, enteritis, thrombocytopenia, and leucopenia and can be fatal in up to 30% of cases. However, the mechanism of severe disease is not fully understood. Molecular imaging approaches, such as positron-emission tomography (PET), are functional in vivo imaging techniques that provide real-time dynamics of disease progression, assessments of pharmacokinetics, and diagnoses for disease progression. Molecular imaging also potentially provides useful approaches to explore the pathogenesis of viral infections. Thus, the purpose of this study was to image the pathological features of SFTSV infection in vivo by PET imaging. In a mouse model, we showed that 18 F-FDG accumulations clearly identified the intestinal tract site as a pathological site. We also demonstrated that 18 F-FDG PET imaging can assess disease progression and response to antiserum therapy within the same individual. This is the first report demonstrating a molecular imaging strategy for SFTSV infection. Our results provide potentially useful information for preclinical studies such as the elucidation of the mechanism of SFTSV infection in vivo and the assessment of drugs for SFTS treatment.