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Evaluation of the performance of differently immobilized recombinant lipase B from Candida antarctica preparations for the synthesis of pharmacological derivatives in organic media

2016; Royal Society of Chemistry; Volume: 6; Issue: 5 Linguagem: Inglês

10.1039/c5ra22508f

2046-2069

Evelin A. Manoel, Julia de Macedo Robert, Martina C. C. Pinto, Antonio Carlos Oliveira Machado, Marina Damião Besteti Ribeiro, Maria Alice Zarur Coelho, Alessandro B. C. Simas, Roberto Fernandéz‐Lafuente, José Carlos Pinto, Denise Maria Guimarães Freire,

Analytical Chemistry and Chromatography

This paper shows the production of lipase B from Candida antarctica (LIPB) after cloning the gene that encoded it in Pichia pastoris using PGK as a constitutive promoter. The lipase was immobilized on different home-made supports for distinct reactions.