Produção Nacional
Revisado por pares
Genetic diversity of HPV16 and HPV18 in Brazilian patients with invasive cervical cancer
2015; Wiley; Volume: 88; Issue: 7 Linguagem: Inglês
10.1002/jmv.24458
ISSN1096-9071
AutoresJoão Paulo Castello Branco Vidal, Shayany Pinto Felix, Cláudia Bessa Pereira Chaves, Patrícia Patury, Vanessa Ferreira Franco, Evaneide A. de Morais, Neile Alves de Carvalho, Aurenice C.L. Carvalho, Olimpio F. Almeida Neto, Lina Maria T.M. Vieira, Flávia de Miranda Corrêa, Luís Felipe Leite Martins, Antonio Negrão, Liz Maria de Almeida, Miguel Ângelo Martins Moreira,
Tópico(s)Reproductive tract infections research
ResumoCervical cancer is the fourth most common cancer among women, and ∼70–80% of these cancers are associated with two human papillomavirus types: HPV16 and HPV18. Several studies have reported that intra‐type diversity is associated with the progression of infection to invasive cancer. Herein, we report the genetic diversity of HPV16 and HPV18 in a cohort of 594 Brazilian women with invasive cervical cancer and describe the prevalence of lineages and intra‐type diversity prior to the implementation of the public immunization program in Brazil. HPV detection and genotyping were performed using PCR, PGMY/GP primers, and DNA extracted from fresh tumors. The HPV16 (378 women) and HPV18 (80 women) lineages were identified by PCR and sequencing of the LCR and E6 fragments, followed by SNV comparison and phylogenetic analysis. In our cohort, was found a higher frequency of the lineage A (in 217 women), followed by lineage D (in 97 women) and lineages B and C (in 10 women each) for HPV16; and a higher frequency of lineage A (in 56 women) followed by lineage B (in 15 women) in HPV18. The genetic diversity of HPV16 indicated a recent expansion of specific variants or a selective advantage that is associated with invasive cancer; this pattern was not observed for HPV18. J. Med. Virol. 88:1279–1287, 2016 . © 2015 Wiley Periodicals, Inc.
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