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WR-2721, Its Derivatives and Their Radioprotective Effects on Mammalian Cells in Culture

1983; Taylor & Francis; Volume: 44; Issue: 1 Linguagem: Inglês

10.1080/09553008314550851

0020-7616

Toshio Mori, Masashi Watanabe, M. Horikawa, P. Nikaido, Hiroshi Kimura, Toshihiro Aoyama, Tsutomu Sugahara,

Plant Genetic and Mutation Studies

Although it is well known that WR-2721 is very efficient in protecting mice against lethal irradiation, we could not find any radioprotective effect of WR-2721 on mouse L cells in culture. But WR-1065 alone (free SH form of WR-2721), and WR-2721 pre-incubated with mouse liver homogenate, showed radioprotective ability. It was found that mouse liver homogenate dephosphorylated WR-2721 to WR-1065. The highest WR-2721 metabolizing activity was found in mouse liver homogenate and Chang liver cell homogenate. Homogenates of human liver and kidney were also shown to possess moderate activity for metabolizing WR-2721. These results suggest to us that WR-2721 must be dephosphorylated before exerting its radioprotective effect and that this dephosphorylating activity varies with tissues. It is demonstrated therefore that mouse L cell in culture is a novel system to assess the extent of dephosphorylation of WR-2721 in various tissues.