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MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL

2015; UNIVERSIDADE DE SÃO PAULO; Volume: 57; Issue: 4 Linguagem: Inglês

10.1590/s0036-46652015000400014

1678-9946

Rafael Felipe da Costa Vieira, Odilon Vidotto, Thállitha Samih Wischral Jayme Vieira, Ana M. S. Guimarães, Andrea Pires dos Santos, Naíla Cannes do Nascimento, Nelson Jessé Rodrigues dos Santos, Thiago Fernandes Martins, Marcelo B. Labruna, Mary Marcondes, Alexander Welker Biondo, Joanne B. Messick,

Viral Infections and Vectors

SUMMARY The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanisalone, 12 ' Candidatus Mycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.