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A Simplified Ultra-Micro Kjeldahl Method for the Estimation of Protein and Total Nitrogen in Fluid Samples. of Less Than 1·0 μ 1

1949; The Company of Biologists; Volume: 26; Issue: 1 Linguagem: Inglês

10.1242/jeb.26.1.15

1477-9145

J. G. Shaw, L. C. Beadle,

thermodynamics and calorimetric analyses

ABSTRACT An ultra-micro modification of the Kjeldahl method is described whereby nitrogen can be estimated in a blood sample of less than 1 μl. Volume. This is simpler than previously described methods as regards apparatus and procedure. The separate estimations of protein and total nitrogen are also introduced. For samples containing 1·0-10·0 μg. N the standard deviation is 0·02-0·04 μg. N. Preliminary tests indicate that for 0·1-1·0 μg. N the standard deviation would be of the order of 0·006-0·008 μg. N.