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A unique duplication of gene cluster ( S 2 – C – Y ) in Epopella plicata (Crustacea) mitochondrial genome and phylogeny within Cirripedia

2015; Taylor & Francis; Volume: 28; Issue: 2 Linguagem: Inglês

10.3109/19401736.2015.1118082

2470-1408

Xin Shen, Ling Ming Tsang, Ka Hou Chu, Benny K. K. Chan,

Coral and Marine Ecosystems Studies

AbstractBarnacles (Crustacea: Cirripedia) are important model species in invertebrate larval biology, intertidal ecology, and anti-fouling researches. The complete mitochondrial genome of the intertidal barnacle Epopella plicata Gray, 1843 (Cirripedia: Sessilia: Tetraclitidae) is presented. The mitochondrial genome of E. plicata of 15 296 bp in length encodes 13 PCGs, 2 rRNA genes, and 25 tRNA genes. The genome of E. plicata has a duplicate gene cluster (trnS2–trnC–trnY) that is unique in the infraclass Cirripedia. The two copies of trnC share identical sequences, but nucleotide substitutions are observed in the other two pairs of tRNAs. Comparison of the two trnS2 indicates that DHU arm and acceptor stem have nucleotide variation. In the two trnY, nucleotide variations are found in the acceptor stem, TψC arm, DHU arm, and variable loop. However, there is no nucleotide variation in the anticodon arm of the three tRNAs. Epopella plicata mitochondrial genome shares seven gene rearrangements with that of Tetraclita japonica (Tetraclitidae), including trnK, trnA, trnE/trnS1, trnP/trnT, trnI/trnQ, trnY and trnC. Comparison of gene orders in the two tetraclitid barnacles and the pancrustacean ground pattern suggests that the arrangement of E. plicata mitochondrial genome is a derived character in this species within the family Tetraclitidae. Sequence analysis of all available barnacle mitochondrial genomes shows that within the order Sessilia, E. plicata and Tetraclita japonica cluster together, resulting in monophyly of Tetraclitidae. Notochthamalus scabrosus (Chthamalidae) is at the basal position of the order Sessilia. Monophyly of the family Balanidae was questioned based on both gene order comparison and sequence analyses, and its phylogenetic status needs to be elucidated further.Keywords: Cirripediagene duplicationmitochondrial genomephylogenyTetraclitidae Declaration of interestThe authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by funding from the Center of Excellence for the Oceans, National Taiwan Ocean University, the Career Development Award from Academia Sinica, Taiwan (AS-98-CDA-L15), grants from the National Science Council, Taiwan (NSC102-2621-B-001-002 to B. K. K. C. and NSC103-2621-B-019-004-MY2 to L. M. T.), the Natural Science Foundation of the Jiangsu Higher Education Institutions (15KJA170001), Open-end Fund of Jiangsu Key Laboratory of Marine Biotechnology (2014HS003), Huaihai Institute of Technology Natural Science Fund (Z2014019), project funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, Project supported by The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Open-end Fund of Jiangsu Institute of Marine Resources (JSIMR201411), Jiangsu 333 and Lianyungang 521 Talent Projects.