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First Report of Onion yellow dwarf virus , Leek yellow stripe virus , and Garlic common latent virus on Garlic in Croatia

2015; American Phytopathological Society; Volume: 100; Issue: 3 Linguagem: Inglês

10.1094/pdis-08-15-0952-pdn

1943-7692

Darko Vončina, Klara Ćurić, Sanja Fabek, N. Tòth,

Plant Disease Resistance and Genetics

HomePlant DiseaseVol. 100, No. 3First Report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus on Garlic in Croatia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus on Garlic in CroatiaD. Vončina, K. Ćurić, S. Fabek, and N. TothD. Vončina, K. Ćurić, S. Fabek, and N. TothAffiliationsAuthors and Affiliations D. Vončina , Department of Plant Pathology K. Ćurić , Student of Graduate Study in Phytomedicine S. Fabek , Department of Vegetable Crops N. Toth , Department of Vegetable Crops, University of Zagreb, Faculty of Agriculture, Croatia. Published Online:15 Jan 2016https://doi.org/10.1094/PDIS-08-15-0952-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Vegetative propagation of garlic often leads to mixed virus infections that cause significant yield and quality reduction. Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), and Garlic common latent virus (GCLV) are the most common viruses in Allium species in the Mediterranean region. They are all transmitted by aphids in a nonpersistant manner. Among these three viruses only OYDV has been previously known to occur in Croatia (Štefanac 1977); however, that report concerned onion infections. At the beginning of 2015, symptoms of yellowing and reduced growth were observed on autochthonous garlic cultivars in commercial fields in Zadar County (northern Dalmatia) and in an experimental field in Zagreb (planting material originating from eastern part of Croatia). A total of 213 symptomatic samples (95 from Zadar County and 118 from Zagreb) were collected and analyzed. Plants were initially tested with ELISA for the presence of OYDV, LYSV, and GCLV using commercial kits (Bioreba AG, Switzerland) following manufacturer's instructions and using leaf tissue as a potential source of antigen. The incidence of viruses varied greatly between the two locations. In samples collected from Zadar County, OYDV infections were significant higher (97.9%) than those of GCLV (11.6%) and LYSV (7.4%). On the other hand, all plants from Zagreb were uniformly infected with a mix of the three targeted viruses. One ELISA-positive plant for each virus was selected for further confirmatory tests. To that purpose, we performed the extraction of total nucleic acid (TNA) using QIAGEN Rneasy Plant mini kit (Valencia, Cam USA) according to manufacturer's instructions. After extraction of TNA, presence of viruses was confirmed by one-step RT-PCR using conditions and primers described by Fidan and Baloglu (2009) for GCLV and Dovas et al. (2001) for OYDV and LYSV, resulting in amplicons of 481, 283, and 304 bp, respectively. PCR products, named GCLV-G-Cro, LYSV-G-Cro, and OYDV-G-Cro, were directly sequenced in both directions (GenBank Accession Nos. KT336495, KT336496, and KT336497, respectively). BLAST searches indicated that Croatian isolates were closest to Japanese isolates of GCLV (AB004804) and OYDV (AB000837), and an Argentinian isolate SW9 of LYSV (KF597284) showing 92%, 96%, and 98% nucleotide identity, respectively. Considering the recent expansion of autochthonous garlic cultivars production, the economic importance of these pathogens is likely to increase. To our knowledge, this is the first report of OYDV, LYSV, and GCLV on garlic in Croatia.References:Dovas, C. I., et al. 2001. J. Phytopathol. 149:731. https://doi.org/10.1046/j.1439-0434.2001.00705.x Crossref, ISI, Google ScholarFidan, H., and Baloglu, S. 2009. J. Plant Pathol. 91(S4):99. Google ScholarŠtefanac, Z. 1977. Acta Bot. Croat. 36:39. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 3 March 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 2 Mar 2016Published: 15 Jan 2016First Look: 19 Oct 2015Accepted: 12 Oct 2015 Page: 656 Information© 2016 The American Phytopathological SocietyCited byLeek yellow stripe virusCABI Compendium, Vol. CABI CompendiumGarlic common latent virusCABI Compendium, Vol. CABI CompendiumOnion yellow dwarf virus (onion yellow dwarf)CABI Compendium, Vol. CABI CompendiumProduction of Virus-Free Garlic Plants through Somatic Embryogenesis29 April 2021 | Agronomy, Vol. 11, No. 5Development of a reverse transcription-recombinase polymerase amplification (RT-RPA) assay for the detection of onion yellow dwarf virus (OYDV) in onion cultivars13 January 2021 | Indian Phytopathology, Vol. 74, No. 1A survey on the infection of Onion yellow dwarf virus and Iris yellow spot tospovirus in seed and bulb productions systems of onion in Calabria, Italy13 January 2020 | European Journal of Plant Pathology, Vol. 156, No. 3First report of onion yellow dwarf virus in Ukraine17 June 2019 | Journal of Plant Pathology, Vol. 101, No. 4Allium sativum (Garlic)6 June 2020