Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
2016; Elsevier BV; Volume: 16; Issue: 2 Linguagem: Inglês
10.1016/j.scr.2015.12.026
ISSN1876-7753
AutoresMaría Questa, Leonardo Romorini, Carolina Blüguermann, Claudia Solari, Gabriel Neiman, Carlos Luzzani, María Élida Scassa, Gustavo Sevlever, Alejandra Guberman, Santiago Miriuka,
Tópico(s)Mesenchymal stem cell research
ResumoHuman foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
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