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MMP-9/RECK Imbalance: A Mechanism Associated with High-Grade Cervical Lesions and Genital Infection by Human Papillomavirus and Chlamydia trachomatis

2015; American Association for Cancer Research; Volume: 24; Issue: 10 Linguagem: Inglês

10.1158/1055-9965.epi-15-0420

1538-7755

Michelle Garcia Discacciati, Fabrícia Gimenes, Paula Comune Pennacchi, Fernanda Faião‐Flores, Luíz Carlos Zeferino, Sophie Derchain, Júlio César Teixeira, Cecília Costa, Marco Antonio Zonta, Lara Termini, Enrique Boccardo, Adhemar Longatto‐Filho, Márcia Edilaine Lopes Consolaro, Luisa L. Villa, Silvya Stuchi Maria‐Engler,

TGF-β signaling in diseases

Abstract Background: Matrix metalloproteinases (MMP) are important enzymes in the tumor microenvironment associated with progression of cervical intraepithelial neoplasia (CIN) toward squamous cell carcinoma (SCC) of the cervix. However, the role of MMPs in the inflammatory process associated with Chlamydia trachomatis infection concomitant with the carcinogenic process driven by HPV has not yet been addressed. In the present study, we analyzed the state of the MMP-9–RECK axis in cervical carcinogenesis. Methods: The levels of MMP-9 and RECK expression were analyzed by immunocytochemistry in liquid-based cytology samples from 136 women with high-grade cervical lesions (CIN2/CIN3) and cervical SCC diagnosed by LLETZ, and in 196 women without cervical neoplasia or CIN1. Real-time qPCR was performed to analyze expression of MMP-9 and RECK in 15 cervical samples. The presence of HPV-DNA and other genital pathogens was evaluated by PCR. Results: We found a higher expression of MMP-9 [OR, 4.2; 95% confidence interval (CI), 2.2–7.8] and lower expression of RECK (OR, 0.4; 95% CI, 0.2–0.7) in women with CIN2/CIN3/SCC when compared with women from the control group (no neoplasia/CIN1). A statistically significant association was also found between MMP-9/RECK imbalance and infection by alpha-9 HPV and C. trachomatis. The prevalence of C. trachomatis infection was significantly higher in women with high-grade cervical disease (OR, 3.7; 95% CI, 1.3–11.3). Conclusions: MMP-9/RECK imbalance in cervical smears is significantly associated with high-grade cervical diseases and infection by alpha-9 HPV and C. trachomatis. Impact: MMP-9/RECK imbalance during cervical inflammation induced by C. trachomatis might play a role in HPV-mediated cervical carcinogenesis. Cancer Epidemiol Biomarkers Prev; 24(10); 1539–47. ©2015 AACR.