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Effects of extracellular purines on ion transport across the integument of Hirudo medicinalis

2002; The Company of Biologists; Volume: 205; Issue: 17 Linguagem: Inglês

10.1242/jeb.205.17.2705

1477-9145

M. Schnizler, Mirjam Buss, Wolfgang Clauss,

Physiological and biochemical adaptations

SUMMARY Little is known about the long-term regulation of epithelial ion transport in invertebrates and the specific mediators involved. For some years, we have been investigating the short-term regulation of transepithelial ion transport across the dorsal integument of the leech Hirudo medicinalis, and we have established a model of Na+ uptake. In the present study, we investigated the effect of long-term acclimation on transintegumental ion transport by adapting leeches to high-salinity conditions. We dissected segments of dorsal integument and measured ion currents in Ussing chamber experiments. Electrophysiological variables, such as transepithelial potential(VT) and short-circuit-current (Isc),were profoundly affected by adaptation to high-salinity conditions. The total transepithelial Na+ current (INa) decreased from 7.66±0.82 to 4.6±0.54 μA cm-2 in preparations adapted to high salinity. The involvement of epithelial Na+channels was determined as current inhibition (Iami) by apical application of amiloride; Na+ channels were equally active in control epithelia and epithelia from leeches adapted to high salinity. Removal of Ca2+ from the apical solutions, which is believed to reduce intracellular Ca2+ concentrations, equalized transepithelial variables between high-salt-adapted integuments and control integuments. Extracellular purines regulate transepithelial Cl- secretion and Na+ absorption. In a variety of tissues we tested ATP and adenosine for their effects on epithelial transport. Examination of integuments from pondwater- and high-salinity-adapted leeches revealed different sensitivities for these purines. Apical and basolateral application of ATP both stimulated transepithelial Na+ uptake and Iami. Adenosine upregulated non-Na+ currents and acted from the basolateral side only. Apical Ca2+-free conditions attenuated these effects of purines on transepithelial currents. Extracellular UTP had no effect on ion transport.