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In Vivo EPR Oximetry Using Two Novel Probes: Fusinite and Lithium Phthalocyanine

1992; Springer Nature; Linguagem: Inglês

10.1007/978-1-4615-3428-0_24

2214-8019

James F. Glockner, Harold M. Swartz,

Insect and Pesticide Research

The concentration of oxygen is an important variable in many physiological and pathological processes; it is not surprising, therefore, that a variety of methods have been developed to measure oxygen tensions in biological systems. Many techniques provide accurate and reliable determinations of in vitro oxygen tensions; in vivo measurements are more difficult, however, and the currently available methodologies have significant limitations. In this study we employ two novel EPR oximetric probes, fusinite and lithium phthalocyanine (LiPc), along with a low frequency (1.1 GHz) electron paramagnetic resonance (EPR) spectrometer and surface probe to measure intramuscular oxygen pressures in anesthetized mice. Principles of in vivo EPR oximetry are discussed in a preceding article by Swartz.