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Regulatory T cells may participate in Helicobacter pylori persistence in gastric MALT lymphoma: lessons from an animal model

2015; Impact Journals LLC; Volume: 7; Issue: 3 Linguagem: Inglês

10.18632/oncotarget.6492

1949-2553

Amandine Laur, Pauline Floch, Lucie Chambonnier, Lucie Bénejat, Victoria Korolik, Alban Giese, Pierre Dubus, Françis Mégraud, António Bandeira, Philippe Lehours,

Galectins and Cancer Biology

// Amandine Marine Laur 1, 2, * , Pauline Floch 1, 2, * , Lucie Chambonnier 1, 2 , Lucie Benejat 1 , Victoria Korolik 3 , Alban Giese 4 , Pierre Dubus 4 , Francis Mégraud 1, 2 , Antonio Bandeira 5 , Philippe Lehours 1, 2 1 University Bordeaux, Bacteriology Laboratory, Bordeaux, France 2 INSERM U853, Bordeaux, France 3 Institute for Glycomics, Griffith University, Nathan QLD, Australia 4 University Bordeaux, EA 2406, Bordeaux, France 5 Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, Paris, France * These authors have contributed equally to this work Correspondence to: Philippe Lehours, e-mail: philippe.lehours@u-bordeaux.fr Keywords: MALT lymphoma, regulatory T cell, animal model, Helicobacter pylori Received: August 04, 2015 Accepted: November 16, 2015 Published: December 07, 2015 ABSTRACT It has been postulated that the emergence of autoimmune gastritis in neonatal thymectomised (d3Tx) BALB/c mice may be a consequence of post-surgery deficit in Tregs. In this study, previously obtained samples from d3Tx mice were used in order to determine whether thymectomy creates a deficit in this T cell subset thereby allowing the emergence of autoimmune phenomena as a prerequisite for GML. The splenic Treg reserve and the local recruitment of these cells in the gastric mucosa were investigated using complementary molecular and immunohistochemistry approaches. Higher Foxp3/CD3 ratios were found in the spleen of non-infected d3Tx mice compared to non-thymectomised (NTx) controls. These results indicate a relative enrichment of Tregs following thymectomy in adult mice. The absence of Treg depletion in d3Tx mice is in line with the absence of auto-immune gastritis in non-infected d3Tx mice. Higher levels of T cell and Treg infiltration were also found in the stomach of GML-developing d3Tx mice versus NTx mice. Surprisingly, inflammatory scores inversely correlated with the bacterial inoculum. The presence of a small Treg containing compartment among gastric biopsies of GML developing d3Tx mice may play a role in perseverance of a minimal bacterial numbers thereby maintaining an antigen-dependent stimulation and proliferation.