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Establishment and Characterization of a Human Primary Prostatic Adenocarcinoma Cell Line (ND-1)

1992; Lippincott Williams & Wilkins; Volume: 148; Issue: 5 Part 1 Linguagem: Inglês

10.1016/s0022-5347(17)36978-1

1527-3792

Perinchery Narayan, Rajvir Dahiya,

Monoclonal and Polyclonal Antibodies Research

No AccessJournal of Urology1 Nov 1992Establishment and Characterization of a Human Primary Prostatic Adenocarcinoma Cell Line (ND-1) Perinchery Narayan, and Rajvir Dahiya Perinchery NarayanPerinchery Narayan , and Rajvir DahiyaRajvir Dahiya View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)36978-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail A human primary prostatic adenocarcinoma cell line named ND-1 has been established in long term tissue culture. The cultured cells show growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice. Cytogenetic analysis by G-banding demonstrated an aneuploid karyotype with a modal chromosome number of 62, and multiple marker chromosomes with 25-30% structural abnormalities. Ploidy analysis revealed that the majority of ND-1 cells (67%) had a DNA mass of 10.1 picogram and DNA index of 1.41. Nineteen percent of cells had a DNA mass of 21.3 picogram and DNA index of 3.0. Electron microscopic studies revealed common features of neoplastic epithelial cells such as numerous microvilli, junctional complexes, abnormal nuclei, nucleoli, and mitochondria. Due to the absence of a structurally normal Y chromosome, confirmation of the presence of a derived Y chromosome was achieved through the use of C-banding and through fluorescent in situ hybridization with a Y chromosome repeat probe. Tandem E PSA immunoenzymatic assay revealed that these ND-1 cells secrete small amounts of prostate specific antigen. © 1992 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited BySOBEL R and SADAR M (2018) CELL LINES USED IN PROSTATE CANCER RESEARCH: A COMPENDIUM OF OLD AND NEW LINES—PART 1Journal of Urology, VOL. 173, NO. 2, (342-359), Online publication date: 1-Feb-2005.DASARI V, DENG D, PERINCHERY G, YEH C and DAHIYA R (2018) DNA METHYLATION REGULATES THE EXPRESSION OF Y CHROMOSOME SPECIFIC GENES IN PROSTATE CANCERJournal of Urology, VOL. 167, NO. 1, (335-338), Online publication date: 1-Jan-2002.LI L, ZHAO H, NAKAJIMA K, OH B, FILHO L, CARROLL P and DAHIYA R (2018) METHYLATION OF THE E-CADHERIN GENE PROMOTER CORRELATES WITH PROGRESSION OF PROSTATE CANCERJournal of Urology, VOL. 166, NO. 2, (705-709), Online publication date: 1-Aug-2001. Volume 148Issue 5 Part 1November 1992Page: 1600-1604 Advertisement Copyright & Permissions© 1992 by The American Urological Association Education and Research, Inc.Keywordsantigenscell lineDNAprostatic neoplasiaMetricsAuthor Information Perinchery Narayan More articles by this author Rajvir Dahiya More articles by this author Expand All Advertisement PDF DownloadLoading ...